Halobacillus andaensis sp nov., a moderately halophilic bacterium isolated from saline and alkaline soil
Halobacillus andaensis sp nov., a moderately halophilic bacterium isolated from saline and alkaline soil
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安达盐杆菌 (Halobacillus andaensis sp nov.)，一种从盐碱土壤中分离出的中度嗜盐细菌
DOI：
10.1099/ijs.0.000198
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发表时间：
2015
影响因子：
2.8
通讯作者：
Jiang Juquan
中科院分区：
文献类型：
--
作者：
Wang Kaibiao;Zhang Lei;Yang Yang;Pan Yuanyuan;Meng Lin;Liu Henan;Hong Shan;Huang Haipeng;Jiang Juquan
从中国安达市盐碱地上分离到一株革兰氏阳性、内孢子形成的中度嗜盐细菌NEU-ST10-40T。它完全是好氧的，杆状，由富周鞭毛活动。在盐浓度为3~15 %(w/v)(最适为8 %，w/v)、pH 为7.0~9.0(最适pH 为8.0)、4~60 °C(最适为30 °C)的条件下生长。细胞壁肽聚糖中含有中位二氨基甲酸。基于16S rRNA基因序列的系统发育分析表明，该菌属于嗜盐杆菌属。菌株NEAU-ST10-40T与盐杆菌属相关种模式菌株的16S rRNA基因序列相似性水平在98.8 %(嗜碱嗜盐杆菌)至97.1 %(黑色盐杆菌)之间。菌株NEAU-ST10-40TH的DNA-DNA杂交关联值。嗜碱杆菌DSM 18525T、野嗜盐杆菌KCTC 13144T、野嗜盐杆菌DSM 17110T、嗜盐嗜盐杆菌DSM 2266T、斜纹盐杆菌DSM 10405T、达班盐杆菌DSM 18199T、盐生盐杆菌DSM 18897T、瑙州盐生盐杆菌DSM 21183T、真嗜盐杆菌DSM 10404T和盐生盐杆菌DSM 21185T的平均值为43 ± 1~19 ± 1 %( ±SD)。DNAG+C含量为39.3 摩尔%。主要脂肪酸(>；10 %)为Anteiso-C15：0、Anteiso-C17：0和iso-C16：0，唯一检测到的呼吸苯二酚是MK-7，极性脂由二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、两种未知磷脂和三种未知脂组成。在此基础上，我们认为菌株NEAU-ST10-40T代表了一个新的种，命名为盐杆菌和盐杆菌。11月是提议的。模式菌株为NEAU-ST10-40T( = CGMCC1.12153T= DSM25866T)。
A Gram-stain-positive, endospore-forming, moderately halophilic bacterial strain, NEAU-ST10-40T, was isolated from a saline and alkaline soil in Anda City, China. It was strictly aerobic, rod-shaped and motile by peritrichous flagella. It formed light yellow colonies and grew at NaCl concentrations of 3–15 % (w/v) (optimum, 8 %, w/v), at pH 7.0–9.0 (optimum, pH 8.0) and at 4–60 °C (optimum, 30 °C). It containedmeso-diaminopimelic acid in the cell-wall peptidoglycan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that it belonged to the genusHalobacillus. Levels of 16S rRNA gene sequence similarity between strain NEAU-ST10-40Tand the type strains of related species of the genusHalobacillusranged from 98.8 % (Halobacillus alkaliphilusFP5T) to 97.1 % (Halobacillus kuroshimensisIS-Hb7T). DNA–DNA hybridization relatedness values between strain NEAU-ST10-40TandH. alkaliphilusDSM 18525T,Halobacillus campisalisKCTC 13144T,Halobacillus yeomjeoniDSM 17110T,Halobacillus halophilusDSM 2266T,Halobacillus litoralisDSM 10405T,Halobacillus dabanensisDSM 18199T,Halobacillus salinusDSM 18897T,Halobacillus naozhouensisDSM 21183T,Halobacillus trueperiDSM 10404TandHalobacillus salsuginisDSM 21185Twere from 43 ± 1 to 19 ± 1 % (mean ±sd). The DNA G+C content was 39.3 mol%. The major fatty acids (>10 %) were anteiso-C15:0, anteiso-C17:0and iso-C16:0, the only respiratory quinone detected was MK-7, and polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unknown phospholipids and three unknown lipids. On the basis of the data presented, strain NEAU-ST10-40Tis considered to represent a novel species, for which the nameHalobacillus andaensissp. nov. is proposed. The type strain is NEAU-ST10-40T( = CGMCC 1.12153T= DSM 25866T).