Influenza virus is not restricted by tetherin whereas influenza VLP production is restricted by tetherin.
Influenza virus is not restricted by tetherin whereas influenza VLP production is restricted by tetherin.
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DOI：
10.1016/j.virol.2011.05.006
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发表时间：
2011-08-15
期刊：
影响因子：
3.7
通讯作者：
Lamb RA
中科院分区：
文献类型：
--
作者：
Watanabe R;Leser GP;Lamb RA
Tetherin（ST 2/CD 317）是一种细胞蛋白，限制包括HIV-1在内的一些包膜病毒从细胞中释放。为了检查流感病毒是否受系链蛋白影响，用流感病毒感染组成型表达人系链蛋白的MDCK细胞和对照MDCK细胞。感染后24小时或48小时，感染滴度无差异。相反，tetherin表达抑制流感病毒样颗粒（VLP）释放到培养基中。HIV蛋白Vpu的表达克服了流感病毒VLP的系链蛋白阻断。构建缺乏C-末端GPI锚附着信号的人系链蛋白突变体（系链蛋白-ΔGPI）以测试该突变体是否可以掺入释放的病毒或VLP颗粒中。尽管系链蛋白-ΔGPI被掺入流感VLP中，但其未被掺入流感病毒体中。总之，这些数据表明，流感病毒粒子可能含有一种连接蛋白拮抗剂。
Tetherin (ST2/CD317) is a cellular protein that restricts the release from cells of some enveloped viruses including HIV-1. To examine if influenza virus is affected by tetherin, MDCK cells constitutively expressing human tetherin and control MDCK cells were infected with influenza virus. No difference was observed in infectious titers, at 24 h or 48 h post-infection. In contrast, tetherin expression inhibited influenza virus-like particle (VLP) release into the media. Expression of the HIV protein Vpu overcame the tetherin block of influenza virus VLPs. A human tetherin mutant that lacks a C-terminal GPI anchor attachment signal (tetherin-ΔGPI) was constructed to test if this mutant could be incorporated into the released virus or VLP particles. Whereas tetherin-ΔGPI was incorporated into influenza VLPs it was not incorporated into influenza virions. Taken together these data suggest that influenza virions may contain a tetherin antagonist.
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