Short interfering RNAs specific for potato spindle tuber viroid are found in the cytoplasm but not in the nucleus
Short interfering RNAs specific for potato spindle tuber viroid are found in the cytoplasm but not in the nucleus
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DOI：
10.1111/j.1365-313x.2004.02001.x
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发表时间：
2004-03-01
期刊：
影响因子：
7.2
通讯作者：
Tabler, M
中科院分区：
文献类型：
--
作者：
Denti, MA;Boutla, A;Tabler, M
短干扰(Si)和微干扰(Mi)RNA在转录后水平影响基因表达。在植物中，不同类别的Dector-like(DCL)酶负责从不同的前体产生这些小的调控RNA。为了确定它们的产生和积累的细胞位置，我们从感染马铃薯纺锤体块茎类病毒(PSTVd)RNA的番茄植株中纯化了细胞核，已知PSTVd RNA通过双链(DS)RNA中间体在细胞核中复制。我们可以在细胞质部分检测到PSTVd特异的siRNAs，但在核部分检测不到。为了将PSTVd特异性siRNA的定位与类似大小的小RNA相关联，我们研究了自然产生的miRNA的区隔。在细胞核中可以检测到miR167的前体，但成熟的miRNA仅存在于细胞质中。我们讨论了这一发现对类病毒复制和异染色质形成模型的影响。
Short interfering (si) and micro (mi) RNAs influence gene expression at post-transcriptional level. In plants, different classes of DICER-LIKE (DCL) enzymes are responsible for the generation of these small regulatory RNAs from different precursors. To characterize the cellular site of their generation and accumulation, we purified nuclei from tomato plants infected with potato spindle tuber viroid (PSTVd) RNA, which is known to replicate in the nucleus via double-stranded (ds) RNA intermediates. We could detect PSTVd-specific siRNAs in the cytoplasmic fraction, but not in the nuclear fraction. To correlate the localization of the PSTVd-specific siRNAs with that of similarly sized small RNAs, we studied the compartmentalization of a naturally occurring miRNA. We could detect the precursor of miR167 in the nucleus, but the mature miRNA was found only in the cytoplasmic fraction. We discuss the consequences of this finding for the model of viroid replication and heterochromatin formation.