Preparation of highly efficient monolithic silica capillary columns for the separations in weak cation-exchange and HILIC modes
Preparation of highly efficient monolithic silica capillary columns for the separations in weak cation-exchange and HILIC modes
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DOI：
10.1016/j.jbbm.2006.09.010
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发表时间：
2007-02-23
影响因子：
--
通讯作者：
Tanaka, Nobuo
中科院分区：
文献类型：
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作者：
Ikegami, Tohru;Horie, Kanta;Tanaka, Nobuo
弱阳离子交换 (WCX) 和 HILIC 模式色谱柱是通过丙烯酸在用 N-(3-三乙氧基甲硅烷基丙基)甲基丙烯酰胺锚定基团修饰的整体式硅胶毛细管柱上进行柱上聚合来制备的。聚合物涂层柱可用于吡啶氨基 (PA)-糖和肽的 HILIC 模式分离，包括 BSA 的胰蛋白酶消化，而用于弱阳离子交换模式，即使在高线速度下也可分离蛋白质和核苷。聚(丙烯酸)涂覆的整体式硅胶毛细管柱比以相同方式制备的聚丙烯酰胺涂覆的整体式硅胶毛细管柱表现出对PA-糖更大的保留。使用同一色谱柱在 pH 6.9 下有效分离蛋白质和核苷。在聚合物涂覆步骤之后，该柱提供了良好的渗透性。事实证明，在 u = 4.66 mm/s 下高效高速分离蛋白质是可能的，而使用 u = 8.67 mm/s 的色谱柱在一分钟内即可实现核苷的高速分离，这表明该色谱柱将适用于多维 HPLC 系统的二维分离。 (c) 2006 Elsevier B.V. 保留所有权利。
Weak cation-exchange (WCX) and HILIC modes columns were prepared by on-column polymerization of acrylic acid on monolithic silica capillary columns modified with N-(3-triethoxysilyipropyl)methacrylamide anchor groups. The polymer-coated columns could be used for HILIC mode separation of pyridylamino (PA)-sugars and peptides including a tryptic digest of BSA, while for weak cation-exchange mode for the separation of proteins and nucleosides even at high linear velocity. The poly(acrylic acid) coated monolithic silica capillary columns showed greater retention toward PA-sugars than a polyacrylamide coated monolithic silica capillary columns prepared in the same manner. Proteins and nucleosides were separated effectively at pH 6.9 using the same column. The column provided fair permeability after the polymer-coating step. High-speed separation of proteins at u = 4.66 mm/s with high efficiency was shown to be possible, while high-speed separation of nucleosides has achieved within one minute using the column at u = 8.67 mm/s, suggesting that the column will be suitable for the second dimension separation of multidimensional HPLC systems. (c) 2006 Elsevier B.V. All rights reserved.