Picomolar detection of thrombin with fiber-optic ball resonator sensor using optical backscatter reflectometry
Picomolar detection of thrombin with fiber-optic ball resonator sensor using optical backscatter reflectometry
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DOI：
10.1016/j.ijleo.2021.166969
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发表时间：
2021-05-06
期刊：
影响因子：
3.1
通讯作者：
Tosi, Daniele
中科院分区：
文献类型：
--
作者：
Ayupova, Takhmina;Shaimerdenova, Madina;Tosi, Daniele
一个球谐振器，定位在光纤的尖端，已被开发作为一个生物传感器的原型检测凝血酶。该装置采用快速、可重复的CO2激光拼接方法，然后进行金溅射和功能化，用于测量各种蛋白质浓度。球谐振器作为回波损耗低于- 50 dB的弱干涉仪，用光学后向散射反射计测量反射光谱。我们在这里报告了一个具有高灵敏度的样品（1273.74 nm/RIU, RIU =折射率单位），它允许在0.4-100 pM范围内检测蛋白质，在对数响应中检测限为1.56 pM。
A ball resonator, positioned on the tip of an optical fiber, has been developed as a biosensor for the prototypic detection of thrombin. The device was fabricated with a fast and repeatable CO2 laser splicing method, followed by gold-sputtering and functionalization for the measurement of various protein concentrations. The ball resonator acts as a weak interferometer with a return loss below - 50 dB, and it is interrogated with an optical backscatter reflectometer measuring the reflection spectrum. We report here a sample presenting high sensitivity (1273.74 nm/RIU, RIU = refractive index units), which allows protein detection in the range 0.4-100 pM, with a limit of detection of 1.56 pM in logarithmic response.