Influence of anticoagulants and storage temperatures on blood counts and mitotic index of blood samples collected for cytogenetic biodosimetry.
Influence of anticoagulants and storage temperatures on blood counts and mitotic index of blood samples collected for cytogenetic biodosimetry.
复制标题
抗凝剂和储存温度对收集用于细胞遗传学生物剂量测定的血液样本的血细胞计数和有丝分裂指数的影响。
DOI：
10.1080/09553002.2019.1539882
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Miura T.
中科院分区：
文献类型：
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作者：
1.Fujishima Y;Kanahama S;Hagino S;Natsubori S;Saito H;Azumaya A;Ariyoshi K;Nakata A;Kasai K;Yamada K;Mariya Y;Yoshida MA;Miura T.
目的：探讨保存温度、保存时间和抗凝剂种类对双着丝粒染色体分析(DCA)所需中期染色体数目的影响。材料与方法：采集5名健康献血员外周血，采用肝素锂和乙二胺四乙酸二钾(EDTA-2K)。用3 GyX射线照射或假照射，将标本分成室温(RT)和5.2 ± 1.0 °C两组，分别保存6、24、48、72和16 8 h后进行血细胞计数和有丝分裂指数(MI)分析。经EDTA处理的血液样本在细胞计数方面没有表现出如此明显的变化。保存6 h后，经RT保存的肝素化血液样品的MI为21.5-29.3%。EDTA洗涤组保存6、24、48和72 h可获得相似的MI。结论：肝素化血样应在RT条件下保存，以获得足够的DCA中期，EDTA血样经5.2 ± 1.0 °C洗涤保存后也可用于血培养。
Purpose:In order to establish suitable protocols of blood culture to obtain sufficient numbers of metaphases for dicentric chromosome assay (DCA), we have examined the effect of storage temperature, storage time, and anticoagulant type.Materials and methods:Peripheral blood was collected from five healthy donors with lithium heparin and ethylenediaminetetraacetic acid dipotassium salt (EDTA-2K). These samples were irradiated with X-rays at 3 Gy or sham; the samples were further divided into groups that were either stored at room temperature (RT) or 5.2 ± 1.0 °C. After 6, 24, 48, 72, and 168 h of storage, both blood counts and the mitotic index (MI) were analyzed.Results:Heparinized blood samples stored under cold conditions exhibited low white blood cell, lymphocyte, and platelet counts. EDTA-treated blood samples did not show such obvious changes in cell counts. After 6 h of storage, heparinized blood samples stored at RT had MI of 21.5–29.3%. Similar MI was obtained in the EDTA-washed group stored for 6, 24, 48, and 72 h.Conclusions:Our study confirms that heparinized blood samples should be stored at RT to get sufficient metaphases for DCA, and that EDTA blood samples also can be used for blood culture after washing and storage under 5.2 ± 1.0 °C.