Evaluation of spatial memory of C57BL/6J and CD1 mice in the Barnes maze, the Multiple T-maze and in the Morris water maze
Evaluation of spatial memory of C57BL/6J and CD1 mice in the Barnes maze, the Multiple T-maze and in the Morris water maze
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DOI：
10.1016/j.bbr.2008.10.029
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发表时间：
2009-03-02
影响因子：
2.7
通讯作者：
Lubec, Gert
中科院分区：
文献类型：
--
作者：
Patil, Sudarshan S.;Sunyer, Berta;Lubec, Gert
空间学习和记忆的评估主要使用Morris水迷宫作为单一范式。我们打算测试Barnes迷宫和多T迷宫中的小鼠是否会导致可比的结果，并测试两个不同焦虑程度的单独品系的小鼠。在获取阶段，使用潜伏期、路径长度、BM中的错误和MTM中的正确决策来测量学习。对小鼠进行为期4天的训练，并在第5天和第12天进行探针试验。训练期间潜伏期的减少表明，两个菌株都学习了所有任务。在第5天和第12天的滞留期，C57BL/6J小鼠在Barnes迷宫和多重T迷宫中的表现优于CD1小鼠，而CD1小鼠在Morris水迷宫中的表现优于C57BL/6J小鼠。在第12天的BM中，C57BL/6J保持了第5天观察到的对目标的访问水平，而CD1表现更差。因此，对空间记忆的公平评估需要应用(至少)两个不同的测试系统，一个是水迷宫，一个是陆地迷宫。不同的焦虑相关行为以及所用品系的压力反应可能有助于解释所报道的结果，并再次建议在考虑到对空间记忆的稳健评估时使用至少两个品系的小鼠。(C)2008爱思唯尔B.V.保留所有权利。
Evaluation of spatial learning and memory is mainly carried out using the Morris water maze as a single paradigm. We intended to test whether mice in the Barnes maze and Multiple T-maze would lead to comparable results and to test two individual mouse strains with different anxiety levels.C57BL/6J and CD1 male mice were used in the experiments. During the acquisition phase, learning was measured using parameters latency, path length, errors in the BM and correct decisions in MTM. Mice were trained for 4 days and probe trials were performed on days 5 and 12.Latencies reduction over the training period indicated that both strains learned all tasks. During retention phase at days 5 and 12 C57BL/6J performed the Barnes maze and Multiple T-maze task better than CD1 mice while CD1 performed better than C57BL/6J in the Morris water maze. In the BM at day 12, C57BL/6J kept the level of visits to target observed at day 5 whereas CD1 performed worse.Strain- and task-dependent differences were observed using the three mazes. Therefore, fair evaluation of spatial memory demands application of (at least) two different test systems, a water- and a land maze. Different anxiety-related behaviour as well as stress-responses in the strains used may help to interpret the findings reported and again may propose the use of at least two mouse strains when robust evaluation of spatial memory is considered. (C) 2008 Elsevier B.V. All rights reserved.