Dietary supplementation with omega-3 polyunsaturated fatty acids robustly promotes neurovascular restorative dynamics and improves neurological functions after stroke.
Dietary supplementation with omega-3 polyunsaturated fatty acids robustly promotes neurovascular restorative dynamics and improves neurological functions after stroke.
复制标题
膳食补充 omega-3 多不饱和脂肪酸可强有力地促进神经血管恢复动力学并改善中风后的神经功能
DOI：
10.1016/j.expneurol.2015.03.005
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发表时间：
2015-10
影响因子：
5.3
通讯作者：
Chen J
中科院分区：
文献类型：
--
作者：
Zhang W;Wang H;Zhang H;Leak RK;Shi Y;Hu X;Gao Y;Chen J
中风是一种毁灭性的神经系统疾病，没有令人满意的治疗方法来保护长期的神经功能，这可能是由于大多数临床前研究只强调神经元的存活。最近的研究表明，在功能长期恢复之前，保护受损大脑中的多种细胞类型（如少突胶质细胞和神经血管单位的成分）的重要性。例如，缺血半暗带中的血管再生对于提供增强神经元和其他祖细胞（如少突胶质细胞前体细胞）的存活和活性的各种神经营养因子至关重要。在本研究中，我们假设长期膳食补充鱼油可促进中风后血管生成、神经发生和少突神经发生，从而导致长期功能改善。小鼠在中风前三个月和中风后一个月接受富含n-3 PUFA的鱼油的饮食补充。正如预期的那样，饮食n-3 PUFAs显著增加了大脑中n-3 PUFAs的水平，并改善了脑缺血后的长期行为结果。n-3 PUFAs还有力地改善了血管再生和血管生成，并提高了中风损伤后NeuN/BrdU标记的新生神经元的存活长达35天。此外，这些促神经原性作用伴随着强大的少突胶质细胞生成。因此，这是第一项证明长期饮食摄入n-3 PUFAs是一种有效的预防措施，不仅可以长期保护缺血性损伤，还可以积极促进神经血管恢复动力学和脑修复。
Stroke is a devastating neurological disease with no satisfactory therapies to preserve long-term neurological function, perhaps due to the sole emphasis on neuronal survival in most preclinical studies. Recent studies have revealed the importance of protecting multiple cell types in the injured brain, such as oligodendrocytes and components of the neurovascular unit, before long-lasting recovery of function can be achieved. For example, revascularization in the ischemic penumbra is critical to provide various neurotrophic factors that enhance the survival and activity of neurons and other progenitor cells, such as oligodendrocyte precursor cells. In the present study, we hypothesized that chronic dietary supplementation with fish oil promotes post-stroke angiogenesis, neurogenesis, and oligodendrogenesis, thereby leading to long-term functional improvements. Mice received dietary supplementation with n-3 PUFA-enriched fish oil for three months before and up to one month after stroke. As expected, dietary n-3 PUFAs significantly increased levels of n-3 PUFAs in the brain and improved long-term behavioral outcomes after cerebral ischemia. n-3 PUFAs also robustly improved revascularization and angiogenesis and boosted the survival of NeuN/BrdU labeled newborn neurons up to 35 days after stroke injury. Furthermore, these pro-neurogenic effects were accompanied by robust oligodendrogenesis. Thus, this is the first study to demonstrate that chronic dietary intake of n-3 PUFAs is an effective prophylactic measure to not only protect against ischemic injury for the long term but also to actively promote neurovascular restorative dynamics and brain repair.