Genetic Prevalence of Porcine Endogenous Retrovirus in Chinese Experimental Miniature Pigs
Genetic Prevalence of Porcine Endogenous Retrovirus in Chinese Experimental Miniature Pigs
复制标题
中国实验小型猪猪内源性逆转录病毒的遗传流行情况
DOI：
10.1016/j.transproceed.2011.06.061
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发表时间：
2011-09-01
影响因子：
0.9
通讯作者：
Gao, Y.
中科院分区：
文献类型：
--
作者：
Liu, G.;Li, Z.;Gao, Y.
猪对人的异种移植存在猪内源性逆转录病毒（PERV）种间传播的潜在风险。中国实验小型猪可作为猪-人异种移植供体。然而，中国实验小型猪群体中缺乏 PERV 原病毒在基因组 DNA 中的分布和 RNA 水平上的 PERV 表达的数据。在本研究中，使用聚合酶链反应（PCR）、实时定量PCR和实时定量逆转录PCR对PERV进行了研究。结果显示，20份猪基因组DNA样本中，基因型分布为PERV-A亚型100%、PERV-B亚型100%、PERV-C亚型30%。基因组DNA中的PERV拷贝数和RNA水平上的PERV表达在个体之间存在显着差异，范围分别为3.95+/-0.14至95.52+/-2.20和3.66+/-0.13至43.03+/-2.50。对于一些个体，基因组DNA中的PERV拷贝数(例如，3.95+/-0.14)和RNA水平上的PERV表达(例如，3.66+/-0.13)较低。这些结果表明中国实验小型猪是异种移植的可能供体。我们的研究结果为选育提供了参考信息，这将有利于这些动物在异种移植研究中的应用。
Pig-to-human xenotransplantation poses the potential risk of interspecies transmission of porcine endogenous retrovirus (PERV). The Chinese experimental miniature pig may be used as a pig-to-human xenograft donor. However, data for the distribution of PERV provirus in genomic DNA and PERV expression at the RNA level for the Chinese experimental miniature pig population are lacking. In this study, PERV was investigated in this regard using polymerase chain reaction (PCR), real-time quantitative PCR, and real-time quantitative reverse transcription PCR. The results showed that the genotype distribution was PERV-A subtype 100%, PERV-B subtype 100%, and PERV-C subtype 30% among 20 pig genomic DNA samples. Both PERV copy number in genomic DNA and PERV expression at the RNA level varied significantly among individuals, ranging from 3.95 +/- 0.14 to 95.52 +/- 2.20 and 3.66 +/- 0.13 to 43.03 +/- 2.50, respectively. For some individuals, the PERV copy number (eg, 3.95 +/- 0.14) in genomic DNA and PERV expression (eg, 3.66 +/- 0.13) at the RNA level were low. These results suggested that the Chinese experimental miniature pig is a possible donor for xenotransplantation. Our results provide reference information for selective breeding, which will benefit the application of these animals for the study of xenotransplantation.