Population genetics of Glossina palpalis palpalis from central African sleeping sickness foci
Population genetics of Glossina palpalis palpalis from central African sleeping sickness foci
复制标题
DOI：
10.1186/1756-3305-4-140
复制
发表时间：
2011-07-18
影响因子：
3.2
通讯作者：
Njiokou, Flobert
中科院分区：
文献类型：
--
作者：
Tanekou, Tresor Tito;Melachio, T. T.;Njiokou, Flobert
背景资料：叶蝉（Glossina palpalis palpalis，双翅目：叶蝉科）广泛分布于西非，是喀麦隆和刚果民主共和国下刚果省昏睡病的主要传播媒介。然而，对其种群结构知之甚少。我们调查了G。p. palpalis种群遗传结构在5个昏睡病focuses（4个在喀麦隆，一个在刚果民主共和国）使用8个微卫星DNA marker.Results：一个强大的隔离距离解释了我们的采样点喀麦隆和刚果民主共和国观察到的大部分种群结构。这里的种群是由占相当宽的区域的panmictic亚群组成，具有很强的隔离距离。有效种群的大小在20 ~ 300只之间，如果密度在120 ~ 2000只/km ~ 2之间，繁殖成虫与亲本的扩散距离在60 ~ 300 m之间。p. palpalis在中非已经证明了随机交配亚群在相当大的地区，因此在差异与西非种群的G。[医]须叶这项研究带来了新的信息隔离的距离在宏观地理范围内，这反过来又带来了有用的信息，如何组织区域舌蝇控制。今后的调查应针对时间抽样，以更准确地衡量人口参数，以帮助病媒控制决策。
Background: Glossina palpalis palpalis (Diptera: Glossinidae) is widespread in west Africa, and is the main vector of sleeping sickness in Cameroon as well as in the Bas Congo Province of the Democratic Republic of Congo. However, little is known on the structure of its populations. We investigated G. p. palpalis population genetic structure in five sleeping sickness foci (four in Cameroon, one in Democratic Republic of Congo) using eight microsatellite DNA markers.Results: A strong isolation by distance explains most of the population structure observed in our sampling sites of Cameroon and DRC. The populations here are composed of panmictic subpopulations occupying fairly wide zones with a very strong isolation by distance. Effective population sizes are probably between 20 and 300 individuals and if we assume densities between 120 and 2000 individuals per km(2), dispersal distance between reproducing adults and their parents extends between 60 and 300 meters.Conclusions: This first investigation of population genetic structure of G. p. palpalis in Central Africa has evidenced random mating subpopulations over fairly large areas and is thus at variance with that found in West African populations of G. p. palpalis. This study brings new information on the isolation by distance at a macrogeographic scale which in turn brings useful information on how to organise regional tsetse control. Future investigations should be directed at temporal sampling to have more accurate measures of demographic parameters in order to help vector control decision.