Use of a Polystyrene-divinylbenzene-based Weakly Acidic Cation-Exchange Resin Column and Propionic Acid as an Eluent in Ion-Exclusion/Adsorption Chromatography of Aliphatic Carboxylic Acids and Ethanol in Food Samples
Use of a Polystyrene-divinylbenzene-based Weakly Acidic Cation-Exchange Resin Column and Propionic Acid as an Eluent in Ion-Exclusion/Adsorption Chromatography of Aliphatic Carboxylic Acids and Ethanol in Food Samples
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DOI：
10.2116/analsci.27.505
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发表时间：
2011-04
影响因子：
1.6
通讯作者：
M. Mori;Takahiro Hironaga;Hiroe Kajiwara;N. Nakatani;Daisuke Kozaki;H. Itabashi;Kazuhiko Tanaka
中科院分区：
文献类型：
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作者：
M. Mori;Takahiro Hironaga;Hiroe Kajiwara;N. Nakatani;Daisuke Kozaki;H. Itabashi;Kazuhiko Tanaka
建立了以聚苯乙烯-二乙烯基苯为基体的弱酸性阳离子交换树脂（PS-WCX）为分离柱，丙酸为洗脱剂，同时测定食品中多价脂肪族羧酸和乙醇的离子排斥/吸附色谱（IEAC）方法。PS-WCX柱很好地分离了酸性洗脱液中的一价、二价和三价羧酸。丙酸作为洗脱液给出了更高的信噪比，并使分析物酸的灵敏电导检测。我们发现最佳分离条件是PS-WCX柱和20 mM丙酸的组合。通过在分离柱之前连接一个短的预柱，并在预柱上连接一个H^+型强酸性阳离子交换树脂，证实了所开发方法的实用性;这是通过将阳离子转化为氢离子来去除食品样品中的阳离子。因此，啤酒，葡萄酒和酱料中的常见羧酸和乙醇成功地分离的方法。
We developed an ion-exclusion/adsorption chromatography (IEAC) method employing a polystyrene-divinylbenzene-based weakly acidic cation-exchange resin (PS-WCX) column with propionic acid as the eluent for the simultaneous determination of multivalent aliphatic carboxylic acids and ethanol in food samples. The PS-WCX column well resolved mono-, di-, and trivalent carboxylic acids in the acidic eluent. Propionic acid as the eluent gave a higher signal-to-noise ratio, and enabled sensitive conductimetric detection of analyte acids. We found the optimal separation condition to be the combination of a PS-WCX column and 20-mM propionic acid. Practical applicability of the developed method was confirmed by using a short precolumn with a strongly acidic cation-exchange resin in the H^+-form connected before the separation column; this was to remove cations from food samples by converting them to hydrogen ions. Consequently, common carboxylic acids and ethanol in beer, wine, and soy sauce were successfully separated by the developed method.