PROLIFERATIVE BEHAVIOR OF DIFFERENTIATING CELLS IN DEVELOPING RAT PAROTID GLAND
PROLIFERATIVE BEHAVIOR OF DIFFERENTIATING CELLS IN DEVELOPING RAT PAROTID GLAND
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DOI：
10.1083/jcb.46.1.81
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发表时间：
1970-01-01
影响因子：
7.8
通讯作者：
SREEBNY, LM
中科院分区：
文献类型：
--
作者：
REDMAN, RS;SREEBNY, LM
取20日龄至100日龄大鼠腮腺组织，进行DNA含量测定，并进行光镜和电镜观察。总DNA和DNA浓度的年龄差异表明，有一个快速的实质细胞增殖，直到出生后25天，之后的速度迅速下降，并有快速增加的细胞大小之间的18和25天。这些发现得到了组织学观察的证实，例如在25日龄之前存在大量有丝分裂像，以及在18至25日龄之间腺泡细胞快速成熟。这些数据表明，大鼠腮腺腺泡细胞包括一个不断扩大的细胞群。光镜和电子显微镜观察一致表明，有丝分裂像的细胞在腺体发育的所有阶段都与其他实质细胞一样分化良好，包括在23和25天龄时观察到的成熟腺泡细胞。这些观察结果支持了这样的观点，即在分化的晚期阶段细胞的分裂在某些器官和组织的生长中可能是重要的。
Parotid glands of litters of rats at age intervals from 20 daysin uteroto 100 days were assayed for DNA content and examined by light- and electron-microscopy. The age differences in total DNA and DNA concentration indicated that there was a rapid rate of proliferation of parenchymal cells until 25 postnatal days, after which the rate declined rapidly, and that there was a rapid increase in cell size between 18 and 25 days. These findings were substantiated by histologic observations, such as the presence of numerous mitotic figures until 25 days of age, and the rapid maturation of the acinar cells between 18 and 25 days. These data suggest that the acinar cells of the rat parotid gland comprise an expanding cell population. Light- and electron-microscopic observations consistently indicated that cells with mitotic figures were about as well differentiated as other parenchymal cells at all stages of gland development, including mature acinar cells observed at ages 23 and 25 days. These observations support the view that the division of cells in advanced stages of differentiation may be important in the growth of certain organs and tissues.