IMMUNOHISTOCHEMICAL STUDIES ON VARIOUS ASPECTS OF GLANDULAR IMMUNOGLOBULIN TRANSPORT IN MAN
IMMUNOHISTOCHEMICAL STUDIES ON VARIOUS ASPECTS OF GLANDULAR IMMUNOGLOBULIN TRANSPORT IN MAN
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DOI：
10.1007/bf01002902
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发表时间：
1977-01-01
期刊：
影响因子：
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通讯作者：
BRANDTZAEG, P
中科院分区：
文献类型：
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作者：
BRANDTZAEG, P
在通过生理盐水提取去除细胞外免疫球蛋白[IG]的人体组织标本中表征了腺体相关免疫细胞群体。在胃肠道和呼吸道腺体附近、小唾液腺和大唾液腺以及哺乳期乳腺中，产生免疫球蛋白A的免疫细胞占显着优势。免疫组化，这些细胞含有二聚体伊加与纳入J链。尽管这种局部伊加的生产，免疫组织化学试验酒精固定标本表明，腺间质通常渗透主要由IgG，其中大部分是明显的血清来源。浆液性腺细胞选择性地传输二聚体伊加，这出现沿着他们的侧边和顶部的细胞质中，而上皮发生的IgG是不太明显，并限于间隙。相同的上皮细胞产生称为分泌组分（SC）的糖蛋白，其对含有J链的二聚体伊加和五聚体IgM表现出特异性亲和力。在盐水提取的组织中，伊加有规律地沿着SC产生细胞的边界保留;这可能反映了上皮细胞膜中局部形成的伊加和SC之间的复合。SC显然充当二聚体伊加的腺体受体，二聚体IgA可能通过吸附胞饮作用进入上皮细胞。共价稳定后，伊加sBD SC复合物被挤出腺腔。免疫组化结果显示，高尔基体区含有游离SC，但不含伊加，而SC在上皮细胞的顶端更易出现IgA相关的特征。五聚体IgM由腺体以类似于二聚体伊加的方式处理，但IgM的局部合成通常可以忽略不计，除了在肠道中。
Gland-associated immunocyte populations were characterized in human tissue specimens from which extracellular immunoglobulins [Ig] were removed by saline extraction. There is a striking preponderance of IgA-producing immunocytes adjacent to glands of the gastrointestinal and respiratory tracts, in minor and major salivary glands, and in lactating mammary glands. Immunohistochemically, these cells contain dimeric IgA with incorporated J chain. Despite this local IgA production, immunohistochemical tests on alcohol-fixed specimens demonstrate that the glandular stroma is normally permeated predominantly by IgG, most of which is obviously serum-derived. The serous glandular cells selectively transmit dimeric IgA, which appears along their lateral borders and apically in the cytoplasm, whereas the epithelial occurrence of IgG is less conspicuous and is restricted to the interstices. The same epithelial cells produce a glycoprotein called the secretory component (SC) which exhibits specific affinity for J chain-containing dimeric IgA and pentameric IgM. In saline-extracted tissue, IgA is retained regularly along the borders of SC-producing cells; this probably reflects complexing between locally formed IgA and SC in the epithelial cell membranes. SC apparently functions as a glandular receptor for dimeric IgA which probably enters the epithelial cells by adsorptive pinocytosis. After covalent stabilization, the IgA.sbd.SC complexes are extruded to the gland lumen. Immunohistochemically, the Golgi zone contains free SC but no IgA, whereas SC occurring more apically in the epithelial cell exhibits characteristics of being IgA-associated. Pentameric IgM is handled by the glands in a way similar to dimeric IgA, but local synthesis of IgM is normally negligible, except in the gut.