High-performance ion chromatography method for separation and quantification of inositol phosphates in diets and digesta
High-performance ion chromatography method for separation and quantification of inositol phosphates in diets and digesta
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DOI：
10.1016/j.jchromb.2009.11.046
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发表时间：
2010-02-01
影响因子：
3
通讯作者：
Poulsen, H. D.
中科院分区：
文献类型：
--
作者：
Blaabjerg, K.;Hansen-Moller, J.;Poulsen, H. D.
建立了一种分离定量饲料、日粮、胃和回肠消化前期猪中肌醇磷酸盐(InsP（2) ~ InsP(6)）的梯度高效离子色谱方法，并进行了验证。在Dionex CarboPac (TM) PA1色谱柱上用1.5 mol L（-1）甲磺酸和水梯度分离InsP2-InsP6。常用盐酸与甲磺酸的交换有两个优点：(1)分离过程中得到的基线几乎是水平的；(2)由于甲磺酸不像盐酸那样具有腐蚀性，所以不需要使用所有的惰性HPIC设备。分离得到的27个磷酸肌醇异构体中的23个。ICP-MS用于分离的异构体中磷的定量，并用于计算每个异构体的校正因子，允许使用InsP(6)作为校准标准。InsP(2) ~ InsP(6)的检出限在0.9 ~ 4.4 mg磷L（-1）范围内。大部分磷酸肌醇的回收率为80 ~ 100%，重复性和再现性的CV分别为1 ~ 17%和1 ~ 14%。分别。(C) 2009 Elsevier B.V.版权所有
A gradient high-performance ion chromatographic method for separation and quantification of inositol phosphates (InsP(2)-InsP(6)) in feedstuffs, diets, gastric and ileal digesta front pigs was developed and validated. The InsP2-InsP6 were separated oil a Dionex CarboPac (TM) PA1 column using a gradient with 1.5 mol L(-1) methanesulfonic acid and water. The exchange of the commonly used HCl with methanesulfonic acid has two advantages: (i) the obtained baseline during the separation is almost horizontal and (ii) it is not necessary to use all inert HPIC equipment as the methanesulfonic acid is not as aggressive as HCl. Twenty-three of the 27 separated inositol phosphate isomers were isolated. ICP-MS was used for quantification of phosphorus in the isolated isomers and used for calculation of correction factors for each isomer allowing InsP(6) to be used as calibration standard. The detection limits for InsP(2)-InsP(6) were in the range of 0.9-4.4 mg phosphorus L(-1). The recovery of the major part of the inositol phosphates was 80-100%, and the CV for repeatability and reproducibility were 1-17% and 1-14%. respectively. (C) 2009 Elsevier B.V. All rights reserved.