Quantitative determination of wine polysaccharides by gas chromatography-mass spectrometry (GC-MS) and size exclusion chromatography (SEC)
Quantitative determination of wine polysaccharides by gas chromatography-mass spectrometry (GC-MS) and size exclusion chromatography (SEC)
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DOI：
10.1016/j.foodchem.2011.08.049
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发表时间：
2012-03-01
期刊：
影响因子：
8.8
通讯作者：
Ayestaran, Belen
中科院分区：
文献类型：
--
作者：
Guadalupe, Zenaida;Martinez-Pinilla, Olga;Ayestaran, Belen
葡萄酒多糖对葡萄酒的许多工艺和质量特性起着重要的作用，因此人们提出了几种定量测定葡萄酒多糖的方法。本研究评价了气相色谱-质谱仪(GC-MS)测定葡萄酒中单糖和多糖族的适用性。首先对影响多糖得率的因素进行了评价，并对GC-MS法进行了表征，并与以往报道的GC-FID法进行了比较。两种方法的重复性和重复性值相似，取值范围在1%到14%之间。经MS测定，所有单糖的检出限均在1.0微克以下，检出限均在1.8微克以下。此外，对整个方法进行了回收研究，对不同的葡萄酒样品进行了绝对回收率在81%~116%之间，考虑到多步骤过程，该方法具有很好的实用价值。用GC-MS和GC-FID法同时测定了三种葡萄酒样品中葡萄酒多糖族的含量，结果无显著差异。最后，采用高分辨体积排阻色谱折射率检测器(HRSEC-RID)测定了葡萄酒多糖的分子质量分布，并估算了它们的整体含量。GC法和HRSEC法测得的多糖值之间的相关性(r=0.746，p<；005)表明，后者虽然不能准确地定量，但可以作为一种快速、简便的方法来估计葡萄酒中的总多糖。(C)2011爱思唯尔有限公司。保留所有权利。
Wine polysaccharides play an important role on a number of technological and quality properties of wines and thus several methods have been proposed for their quantification. The present study evaluates the suitability of gas chromatography with mass spectrometry detector (GC-MS) for determining the content of wine monosaccharides and thus polysaccharide families. Factors affecting the yield of polysaccharide precipitation were firstly evaluated and the GC-MS method was characterised and compared with the previously reported GC-FID method. Repeatability and reproducibility values were similar in both methods, with values ranging from 1% to 14%. LODs obtained by MS were below 1.0 mu g for all monosaccharides and LOQs were below 1.8 mu g. Moreover, a recovery study of the whole method was carried out and it provided absolute recoveries between 81% and 116% for different wine samples, very good values taking into account the multi-step procedure. Both GC-MS and GC-FID were applied to determine the content of wine polysaccharide families in three wine samples and no significant differences were observed. Finally, high-resolution size exclusion chromatography with refractive index detector (HRSEC-RID) was applied to obtain the molecular weight distributions of the wine polysaccharides and to estimate their global content. The correlation observed between the polysaccharide values obtained with the GC method and the HRSEC-RID method (r = 0.746, p < 0.05) indicated that the latter could serve as a rapid and simple method to give an estimation of total wine polysaccharides although it can not be used to quantify in a precise way. (C) 2011 Elsevier Ltd. All rights reserved.