A RAPID STAINING METHOD FOR DETECTION OF ENDOPHYTIC FUNGI IN TURF AND FORAGE GRASSES
A RAPID STAINING METHOD FOR DETECTION OF ENDOPHYTIC FUNGI IN TURF AND FORAGE GRASSES
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DOI：
10.1094/phyto-78-237
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发表时间：
1988-02-01
期刊：
影响因子：
3.2
通讯作者：
JOHNSONCICALESE, JM
中科院分区：
文献类型：
--
作者：
SAHA, DC;JACKSON, MA;JOHNSONCICALESE, JM
由于对内生菌感染的禾本科植物的兴趣日益增加，一种有效的检测方法被认为是必要的。为了便于筛选大量的个体种子和植物，开发了一种快速、安全的虎红染色程序。以前的方法是耗时的，往往需要小心煮沸的植物组织。虎红染色法简便、有效、直观效果好。即使在厚的组织中，30-60 ec的最小染色时间也能得到良好的真菌分化，并且不需要额外的固定。种子和干燥组织也被有效染色。
Because of the increased interest in endophyte-infected grasses, an effcient detection method was deemed necessary. To facilitate screening a large number of indiviudal seeds and plants, a rapid, safe staining procedure was developed using rose bengal. Previous methods were time-consuming and often required careful boiling of the plant tissues. The rose bengal staining procedure is simple and effective and gave excellent visual result. Minimal staining times of 30-60 ec gave good fungal differentiation even in thick tissues, and no additional fixing was necessary. Seeds and dried tissue also were stained effectively.