Smart gold nanoparticles enhance killing effect on cancer cells
Smart gold nanoparticles enhance killing effect on cancer cells
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智能金纳米粒子增强对癌细胞的杀伤作用
DOI：
10.3892/ijo.2012.1721
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发表时间：
2013-02-01
影响因子：
5.2
通讯作者：
Kong, Beihua
中科院分区：
文献类型：
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作者：
Song, Kun;Xu, Peng;Kong, Beihua
本研究探讨了金纳米颗粒（GNP）和葡萄糖加帽的 GNP（Glu-GNP）的细胞摄取动力学、亚细胞定位和内化机制。还评估了 GNP 的癌症放射治疗增强作用。我们通过播种技术合成了 GNP 和 Glu-GNP。使用两种人类癌细胞系（HeLa 和 MCF-7）研究了较低剂量的 GNP 和 Glu-GNP 对细胞摄取的影响以及诱导的放射增敏作用。通过透射电子显微镜（TEM）分析纳米粒子的细胞内位置。通过流式细胞术和 TUNEL 测定对基于 GNP 的放射治疗后的细胞凋亡进行分析。癌细胞比裸露的 GNP 吸收更多的 Glu-GNP，并且吸收曲线显示大小和细胞依赖性的吸收。 GNPs 主要位于细胞质中，内吞作用是 GNPs 和 Glu-GNPs 内化的机制。尽管细胞凋亡率没有改变，但较低剂量的 GNP 和 Glu-GNP 仍然增强了 X 射线照射的杀伤效果。本研究的结果证明 Glu-GNP 在肿瘤靶向诊断和治疗方面可能具有光明的前景。
The present study explored the cellular uptake dynamics, the subcellular location and the internalization mechanisms of gold nanoparticles (GNPs) and glucose-capped GNPs (Glu-GNPs). The cancer radiotherapy-enhancing effects of GNPs were also evaluated. We synthesized the GNPs and Glu-GNPs by the seeding technique. The effects on cellular uptake and the radiosensitizing effect induced by GNPs and Glu-GNPs at lower doses were investigated using two human cancer cell lines (HeLa and MCF-7). The intracellular location of the nanoparticles was analyzed by transmission electron microscopy (TEM). Analysis of cellular apoptosis following GNP-based radiotherapy was performed by flow cytometry and TUNEL assay. Cancer cells took up more Glu-GNPs than naked GNPs and the uptake curve showed size- and cell-dependent uptake. GNPs were mainly located in the cytoplasm and endocytosis is the mechanism behind the internalization of GNPs and Glu-GNPs. Lower doses of GNPs and Glu-GNPs still enhanced the killing effect using X-ray irradiation, although the apoptotic rate was not altered. The results presented in this study provide evidence that Glu-GNPs may have a bright future in tumor-targeted diagnosis and treatment.