A nonselective analysis of SV40 transformation of mouse 3T3 cells.
A nonselective analysis of SV40 transformation of mouse 3T3 cells.
复制标题
小鼠 3T3 细胞 SV40 转化的非选择性分析。
DOI：
10.1016/0042-6822(74)90457-7
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发表时间：
1974
期刊：
影响因子：
3.7
通讯作者：
R. Pollack
中科院分区：
文献类型：
--
作者：
R. Risser;R. Pollack
经猴病毒40转化的小鼠细胞在体外表现出许多生长特性的变化。为了探讨这些变化的坐标性质，我们分析了随机选择的40个菌落感染3T3细胞后产生的生长特性。从这些菌落中建立的细胞克隆的特点是密度达到饱和，在10%和1%的牛血清中倍增，在甲基纤维素悬浮液中生长，在正常细胞单层上形成菌落，并且存在病毒抗原。该分析显示，只有5个克隆与3T3细胞无法区分；其余35个克隆与3T3细胞的不同之处在于，它们在1%的小牛血清中生长速度与标准SV40转化细胞一样快。在这35个克隆中，有10个符合先前描述的标准变形子。另外10个表现出介于3T3细胞和标准转化子之间的其他生长特性。这些中间克隆的病毒t抗原水平低于标准转化体，并且在细胞间的染色中表现出相当大的异质性。其余15个克隆t抗原阴性，其饱和密度略高于3T3细胞。细胞行为的这些变化在再克隆时是稳定的。
Mouse cells transformed by simian virus 40 show many alterations in their growth propertiesin vitro. In order to investigate the coordinate nature of these changes, we have analyzed the growth properties of 40 randomly selected colonies arising after SV40 infection of 3T3 cells. Clones of cells, established from these colonies, were characterized as to saturation density and doubling time in 10% and 1% calf serum, growth in methyl cellulose suspension, colony formation on monolayers of normal cells, and presence of viral antigens. This analysis revealed that only 5 of the clones were indistinguishable from 3T3 cells; the remaining 35 clones differed from 3T3 cells in that they grew as rapidly in 1% calf serum as standard SV40 transformed cells. Of these 35 clones, ten corresponded to standard transformants previously described. Another ten showed other growth properties intermediate between 3T3 cells and standard transformants. These intermediate clones had lower levels of viral T-antigen than standard transformants and showed considerable heterogeneity in staining from cell to cell. The remaining 15 clones were T-antigen negative and had saturation densities slightly higher than that of 3T3 cells. These changes in cellular behavior are stable on recloning.