Golgi‐localized MORN1 promotes lipid droplet abundance and enhances tolerance to multiple stresses in Arabidopsis
Golgi‐localized MORN1 promotes lipid droplet abundance and enhances tolerance to multiple stresses in Arabidopsis
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高尔基体本地化的 MORN1 促进脂滴丰度并增强拟南芥对多种胁迫的耐受性
DOI：
10.1111/jipb.13498
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发表时间：
2023
影响因子：
11.4
通讯作者：
Hua, Jian
中科院分区：
文献类型：
--
作者：
Li, Zhan;Gao, Yue;Yan, Jiapei;Wang, Shuai;Wang, Shu;Liu, Yuanyuan;Wang, Shaokui;Hua, Jian
营养组织中的脂滴（LD）最近被认为与植物的环境响应有关，但其调节及其在逆境耐受中的功能还没有很好的了解。在这里，我们通过拟南芥全基因组关联研究，确定了一个膜占据和识别连接1（MORN 1）基因作为胁迫耐受性自然变异的贡献者。其功能缺失突变体和天然变体的表征表明，MORN 1基因是植物生长、抗病性、耐寒性和耐热性的正调节因子。MORN 1蛋白与高尔基体相关，也与LD部分相关。破坏这些关联的蛋白质截短废除了MORN 1蛋白的生物学功能。此外，MORN 1基因是LD丰度的正调控因子，其在LD数量调控和胁迫耐受性中的作用是高度相关的。因此，本研究确定MORN 1作为LD丰度的正调节因子，并有助于胁迫耐受性的自然变化。这暗示了高尔基体在LD生物发生中的潜在参与，并强烈表明LD对植物生长和胁迫反应的不同过程的贡献。
Lipid droplet (LD) in vegetative tissues has recently been implicated in environmental responses in plants, but its regulation and its function in stress tolerance are not well understood. Here, we identified aMembrane Occupation and Recognition Nexus 1(MORN1) gene as a contributor to natural variations of stress tolerance through genome‐wide association study inArabidopsis thaliana. Characterization of its loss‐of‐function mutant and natural variants revealed that theMORN1gene is a positive regulator of plant growth, disease resistance, cold tolerance, and heat tolerance. The MORN1 protein is associated with the Golgi and is also partly associated with LD. Protein truncations that disrupt these associations abolished the biological function of the MORN1 protein. Furthermore, theMORN1gene is a positive regulator of LD abundance, and its role in LD number regulation and stress tolerance is highly linked. Therefore, this study identifies MORN1 as a positive regulator of LD abundance and a contributor to natural variations of stress tolerance. It implicates a potential involvement of Golgi in LD biogenesis and strongly suggests a contribution of LD to diverse processes of plant growth and stress responses.