Improved end-column amperometric detection for capillary electrophoresis
Improved end-column amperometric detection for capillary electrophoresis
复制标题
DOI：
10.1016/0021-9673(96)00238-5
复制
发表时间：
1996-08-23
影响因子：
4.1
通讯作者：
Matysik, FM
中科院分区：
文献类型：
--
作者：
Matysik, FM
本文介绍了一种用于毛细管电泳柱端检测的安培检测器。研究了高压对探测性能的影响。作为这些研究的结果，它表明，端柱检测策略操作高达50 μ m的内径毛细管尺寸。而不使用任何电场倍增器设计。由高压电场的存在引起的工作电极电位的偏移通过经由恒电位仪选择更正的电位设置来补偿。检测器性能的特征在于可达到的分离效率（高香草酸的300 000理论塔板）、检测限（5 . 10-（8）mol/l）和库仑效率（高香草酸为8.9%）。与使用微盘电极，而不是碳纤维电极的实际方面得到解决。
An amperometric detector for end-column detection in capillary electrophoresis is described. The influence of the high voltage on the detection performance is studied. As a consequence of these studies it is shown that the end-column detection strategy operates up to capillary dimensions of 50 mu m I.D. without using any electrical-field decoupler design. The shift of the working electrode potential resulting from the presence of the high voltage electrical field is compensated by selecting a more positive potential setting via the potentiostat.The detector performance is characterized in terms of attainable separation efficiencies (300 000 theoretical plates for homovanillic acid), limits of detection (5 . 10-(8) mol/l for serotonin) and coulometric efficiencies (8.9% for homovanillic acid). Practical aspects associated with the use of microdisk electrodes instead of carbon fiber electrodes are addressed.