Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
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枯草芽孢杆菌 spc-alpha 区域的遗传和转录组织。
DOI：
10.1016/0378-1119(95)00757-1
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
Price,CW
中科院分区：
文献类型：
--
作者：
Suh,JW;Boylan,SA;Oh,SH;Price,CW
我们使用染色体步移方法从枯草芽孢杆菌 (Bs) 的主要核糖体蛋白 (r 蛋白) 基因簇中分离出 10.8 kb 的区域。该区域的基因顺序由基因产物给出，为 r-蛋白 L16-L29-S17-L14-L24-L5-S14-S8-L6-L18-S5-L30-L15-SecY-腺苷酸激酶 (Adk)-甲硫氨酸氨肽酶 (Map)-起始因子 1 (IF1)-L36-S13-S11-RNA 亚基聚合酶-L17。因此，克隆的区域包含大肠杆菌 (Ec) S10 操纵子最后三个基因的同源物，以及整个 spc 和 α 操纵子。该 Bs 组织与 Ec 中相应区域的不同之处在于，在编码 SecY 和 L36 的基因之间包含编码 Adk、Map 和 IF1 的基因。质粒整合实验表明，所有 22 个基因均包含由位于编码 r 蛋白 L16 的基因上游的主要启动子控制的单个大转录单位。启动子探针实验发现这个大转录单元（secY 和图谱启动子）内部的活性较低。 secY 启动子区 (psecY) 包含两种活性，每种活性主要在需要高蛋白输出时的稳定生长期发挥作用。因此，Bs S10-spc-α 区域在遗传和转录组织方面都不同于其 Ec 对应区域。鉴于转录组织的这种差异，Ec 和 Bs 之间协调翻译装置表达的机制也可能不同
We used chromosomal walking methods to isolate a 10.8-kb region from the major ribosomal protein (r-protein) gene cluster of Bacillus subtilis (Bs). The gene order in this region, given by gene product, was r-proteins L16-L29-S17-L14-L24-L5-S14-S8-L6-L18-S5-L30-L15-SecY-adenylate kinase (Adk)-methionine aminopeptidase (Map)-initiation factor 1 (IF1)-L36-S13-S11-α subunit of RNA polymerase-L17. The region cloned, therefore, contains the homologues for the last three genes of the Escherichia coli (Ec) S10 operon, together with entire spc and α operons. This Bs organization differs from the corresponding region in Ec by the inclusion of the genes encoding Adk, Map and IF1 between the genes encoding SecY and L36. Plasmid integration experiments indicated that all 22 genes comprise a single large transcriptional unit controlled from a major promoter which lies upstream from the gene encoding r-protein L16. Promoter probe experiments located lesser activities internal to this large transcriptional unit, the secY and map promoters. The secY promoter region (psecY) contained two activities, each principally functioning in the stationary growth phase when high protein export is required. Thus, the Bs S10-spc-α region differs from its Ec counterpart in both genetic and transcriptional organization. Given this difference in transcriptional organization, the mechanisms coordinating expression of the translational apparatus are also likely to differ between Ec and Bs