Achieving Stable Electrospray Ionization Mass Spectrometry Detection from Microfluidic Chips.
Achieving Stable Electrospray Ionization Mass Spectrometry Detection from Microfluidic Chips.
复制标题
通过微流控芯片实现稳定的电喷雾电离质谱检测。
DOI：
10.1007/978-1-4939-8964-5_15
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Lazar,IuliaM
中科院分区：
文献类型：
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作者：
Lazar,IuliaM
在过去的二十年中，微流控装置作为生物分子分析的生物分析平台的发展取得了显着进展。在这些设备上实现质谱（MS）检测系统已成为必然，并开发了各种芯片-MS电离接口。由于电喷雾离子化（ESI）对于大生物分子（例如蛋白质或肽）的分析特别相关，因此努力集中于推进满足通常在MS检测之前使用的纳米分离技术的需求的界面。然而，实现稳定的ESI条件，使灵敏的MS检测是不平凡的，特别是当喷雾是从一个微制造的平台产生。本章的目的是提供一个逐步的协议，用于生产稳定和有效的电喷雾样品电离微流控芯片，用于毛细管电泳（CE）分离。
The past two decades have witnessed remarkable advances in the development of microfluidic devices as bioanalytical platforms for the analysis of biological molecules. The implementation of mass spectrometry (MS) detection systems on these devices has become inevitable, and various chip-MS ionization interfaces have been developed. As electrospray ionization (ESI) is particularly relevant for the analysis of large biological molecules such as proteins or peptides, efforts have focused on advancing interfaces that meet the demands of nano-separation techniques that are typically used prior to MS detection. Achieving stable ESI conditions that enable sensitive MS detection is, however, not trivial, especially when the spray is generated from a microfabricated platform. This chapter is aimed at providing a step-by-step protocol for producing stable and efficient electrospray sample ionization from microfluidic chips that are used for capillary electrophoresis (CE) separations.