Studying anti-oxidative properties of inclusion complexes of α-lipoic acid with γ-cyclodextrin in single living fission yeast by confocal Raman microspectroscopy
Studying anti-oxidative properties of inclusion complexes of α-lipoic acid with γ-cyclodextrin in single living fission yeast by confocal Raman microspectroscopy
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DOI：
10.1016/j.saa.2018.02.011
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发表时间：
2018-05-15
影响因子：
4.4
通讯作者：
Yamamoto, Tatsuyuki
中科院分区：
文献类型：
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作者：
Noothalapati, Hemanth;Ikarashi, Ryo;Yamamoto, Tatsuyuki
α-硫辛酸（ALA）是有氧代谢中许多酶复合物的必需辅因子，特别是在发生呼吸的真核细胞的线粒体中。它还具有优异的抗氧化性能。该酸有两种立体异构体，R-和S-硫辛酸（R-LA和S-LA），但只有R-LA具有生物学意义，并且仅在我们的体内产生。分裂酵母的突变株Delta dpsl不能合成辅酶Q10，这是酵母呼吸过程中必不可少的，导致氧化应激。因此，其在基本培养基中显示生长延迟。我们研究了ALA的抗氧化性能，在其游离形式和它们的包合物与γ-环糊精使用这种突变酵母模型。这两种游离形式的R-和S-LA以及1：1包合物与γ-环糊精恢复生长的三角洲dps 1取决于浓度和形式。但对野生型裂殖酵母菌株的生长完全没有影响。采用拉曼显微光谱技术在分子水平上了解其抗氧化性能。在1602 cm（-1）处的灵敏拉曼谱带被监测，有和没有添加ALA。发现0.5 mM和1.0 mM浓度的ALA在游离和包合形式中具有相似的效果。在2.5 mM ALA时，游离形式抑制生长，而包合物有助于恢复。5.0 mM ALA显示出抑制作用，与形式无关。我们的研究结果表明，在1602 cm（-1）的拉曼带是一个很好的衡量裂变酵母的氧化应激。(C)2018 Elsevier B. V.版权所有。
alpha-lipoic acid (ALA) is an essential cofactor for many enzyme complexes in aerobic metabolism, especially in mitochondria of eukaryotic cells where respiration takes place. It also has excellent anti-oxidative properties. The acid has two stereo-isomers, R- and S-lipoic acid (R-LA and S-LA), but only the R-LA has biological significance and is exclusively produced in our body. A mutant strain of fission yeast, Delta dpsl, cannot synthesize coenzyme Q10, which is essential during yeast respiration, leading to oxidative stress. Therefore, it shows growth delay in the minimal medium. We studied anti-oxidant properties of ALA in its free form and their inclusion complexes with gamma-cyclodextrin using this mutant yeast model. Both free forms R- and S-LA as well as 1:1 inclusion complexes with gamma-cyclodexhin recovered growth of Delta dps1 depending on the concentration and form. However, it has no effect on the growth of wild type fission yeast strain at all. Raman microspectroscopy was employed to understand the anti-oxidant property at the molecular level. A sensitive Raman band at 1602 cm(-1) was monitored with and without addition of ALAs. It was found that 0.5 mM and 1.0 mM concentrations of ALAs had similar effect in both free and inclusion forms. At 2.5 mM ALAs, free forms inhibited the growth while inclusion complexes helped in recovered. 5.0 mM ALA showed inhibitory effect irrespective of form. Our results suggest that the Raman band at 1602 cm(-1) is a good measure of oxidative stress in fission yeast. (C) 2018 Elsevier B.V. All rights reserved.