Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Viruslike Particle Assays
Modeling the Ebolavirus Life Cycle with Transcription and Replication-Competent Viruslike Particle Assays
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DOI：
10.1007/978-1-4939-7116-9_9
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Hoenen, Thomas
中科院分区：
文献类型：
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作者：
Biedenkopf, Nadine;Hoenen, Thomas
埃博拉病毒是严重出血热的病原体，病死率高，目前尚无批准的特异性治疗方法。作为生物安全4级（BSL 4）制剂，与活埃博拉病毒有关的工作仅限于最大限度的封闭实验室。转录和复制能力病毒样颗粒（trVLP）系统是基于反向遗传学的生命周期建模系统，使研究人员能够在最大限度的遏制实验室之外模拟几乎整个埃博拉病毒的生命周期。这些系统可用于剖析病毒的生命周期，从而增加我们对病毒生物学的理解，以及更多的应用，如筛选和开发新的抗病毒药物，因此代表了埃博拉病毒工作的强大工具。
Ebolaviruses are the causative agent of a severe hemorrhagic fever with high case fatality rates, for which no approved specific therapy is available. As biosafety level 4 (BSL4) agents, work with live ebolaviruses is restricted to maximum containment laboratories. Transcription and replication-competent viruslike particle (trVLP) systems are reverse genetics-based life cycle modeling systems that allow researchers to model virtually the entire ebolavirus life cycle outside of a maximum containment laboratory. These systems can be used to dissect the virus life cycle, and thus increase our understanding of virus biology, as well as for more applied uses such as the screening and development of novel antivirals, and thus represent powerful tools for work on ebolaviruses.