[Intervention of acupuncture on abnormal spatiotemporal pattern of visual cortex area 17 neuron in monocular deprivation amblyopia rats].
[Intervention of acupuncture on abnormal spatiotemporal pattern of visual cortex area 17 neuron in monocular deprivation amblyopia rats].
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DOI：
10.13703/j.0255-2930.2017.01.015
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发表时间：
2017-01
期刊：
影响因子：
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通讯作者：
Tiantian Zhu;Chong-bing Ma;Xingke Yan
中科院分区：
文献类型：
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作者：
Tiantian Zhu;Chong-bing Ma;Xingke Yan
目的：初步探讨针刺对视觉皮层可塑性变化的电生理机制。
方法：将50只14日龄Wistar大鼠随机分为空白组、模型组、早期针刺组、中期针刺组和晚期针刺组，每组10只。除空白组外，其余各组大鼠采用眼睑缝合的方法建立单眼剥夺性弱视模型。模型建立后，空白组和模型组大鼠每天抓取但不进行任何干预。针刺组大鼠在模型建立后的第3、12和21天针刺双侧“睛明”（BL1）、“攒竹”（BL2）、“风池”（GB20）和“光明”（GB37），留针10分钟，每天1次，共9天。治疗后，采用M - NEMEA技术测量各组视觉皮层17区神经元的放电幅度和功率谱密度。
结果：与空白组相比，模型组视觉皮层17区放电神经元数量减少，幅度显著降低（均P<0.05）。与模型组相比，早期针刺组和中期针刺组放电神经元数量增加，幅度显著升高（均P<0.05）。早期针刺组神经元放电幅度高于中期针刺组和晚期针刺组（均P<0.05）；中期针刺组神经元放电幅度高于晚期针刺组（P<0.05）。在采集的120秒时域内，空白组功率谱密度分布主要在-105 dB至-100 dB之间；与空白组相比，模型组功率谱密度增加，分布在-132 dB至-124 dB之间；与模型组相比，针刺组功率谱密度降低，早期针刺组分布在-115 dB至-110 dB之间，中期针刺组分布在-120 dB至-115 dB之间，晚期针刺组分布在-129 dB至-122 dB之间。
结论：单眼剥夺大鼠视觉皮层17区存在时空模式的异常变化，但针刺可调节神经元的异常时空模式。因此，视觉皮层神经元在敏感期具有发育可塑性，早期治疗是取得良好疗效的关键。
OBJECTIVE To preliminarily explore the electrophysiological mechanism of acupuncture on plasticity variability of visual cortex. METHODS Fifty 14-day-old Wistar rats were randomly divided into a blank group, a model group, an early-stage acupuncture group, a middle-stage acupuncture group and a late-stage acupuncture group, 10 rats in each one. Except the blank group, the rats in the remaining groups were treated with eyelid suture to establish the model of monocular deprivation amblyopia. After model establishment, the rats in the blank group and model group were grasped without any intervention everyday. The rats in the acupuncture groups were treated with acupuncture at bilateral "Jingming" (BL 1), "Cuanzhu" (BL 2), "Fengchi" (GB 20) and "Guangming" (GB 37) on the 3rd, 12th and 21st days into model establishment. The needles were retained for 10 min, once a day for totally 9 days. After treatment, the M-NEMEA technology was applied to measure the discharge amplitude and power spectral density of visual cortex area 17 neuron of each group. RESULTS Compared with the blank group, the number of discharged neuron of visual cortex area 17 was reduced, and the amplitude was significantly reduced in the model group (both P<0.05). Compared with the model group, the numbers of discharged neuron were increased, and the amplitudes were significantly increased in the early-stage acupuncture group and middle-stage acupuncture group (all P<0.05). The neuron discharge amplitude in the early-stage acupuncture group was higher than those in the middle-stage acupuncture group and late-stage acupuncture group (both P<0.05); the neuron discharge amplitude in the middle-stage acupuncture group was higher than that in the late-stage acupuncture group (P<0.05). In the 120-second time domain of acquisition, the distribution of power spectral density in the blank group was mainly between -105 dB and -100 dB; compared with the blank group, the power spectral density in the model group was increased and distributed between -132 dB and -124 dB; compared with the model group, the power spectral densities in the acupuncture groups was reduced and distributed between -115 dB and -110 dB in the early-stage acupuncture group, -120 dB and -115 dB in the middle-stage acupuncture group and -129 dB and -122 dB in the late-stage acupuncture group. CONCLUSIONS There are abnormal changes of spatiotemporal pattern in visual cortex area 17 of monocular deprivation rats, but acupuncture can regulate abnormal spatiotemporal pattern of neuron. Therefore, there is developmental plasticity of visual cortex neuron in the sensitive period, and early treatment is key to superior efficacy.