bZIP transcription factor zip-2 mediates an early response to Pseudomonas aeruginosa infection in Caenorhabditis elegans
bZIP transcription factor zip-2 mediates an early response to Pseudomonas aeruginosa infection in Caenorhabditis elegans
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DOI：
10.1073/pnas.0914643107
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发表时间：
2010-02-02
影响因子：
11.1
通讯作者：
Troemel, Emily R.
中科院分区：
文献类型：
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作者：
Estes, Kathleen A.;Dunbar, Tiffany L.;Troemel, Emily R.
关于动物是如何区分病原体和无害微生物的，我们知之甚少。为了解决这个问题，我们研究了线虫感染反应基因的诱导。我们关注的是在C.通过感染细菌病原体铜绿假单胞菌，但不是由同基因减毒gacA突变体诱导的。这些基因中的大多数是独立于已知的免疫途径诱导的。我们为这些基因之一，感染反应基因1（irg-1），产生了一个GFP报告基因，它被野生型铜绿假单胞菌菌株PA 14强烈诱导，但不被其他C。elegans病原体或对C.优雅的为了鉴定感染后诱导irg-1的途径的组成部分，我们对C.线虫转录因子该筛选鉴定了zip-2，一种诱导irg-1所需的bZIP转录因子，以及其他几个基因，并且对于防御铜绿假单胞菌感染很重要。这些数据表明，zip-2是由铜绿假单胞菌的强毒株诱导的特异性病原体应答途径的一部分，并提供针对该病原体的防御。
Very little is known about how animals discriminate pathogens from innocuous microbes. To address this question, we examined infection-response gene induction in the nematode Caenorhabditis elegans. We focused on genes that are induced in C. elegans by infection with the bacterial pathogen Pseudomonas aeruginosa, but are not induced by an isogenic attenuated gacA mutant. Most of these genes are induced independently of known immunity pathways. We generated a GFP reporter for one of these genes, infection response gene 1 (irg-1), which is induced strongly by wildtype P. aeruginosa strain PA14, but not by other C. elegans pathogens or by other wild-type P. aeruginosa strains that are weakly pathogenic to C. elegans. To identify components of the pathway that induces irg-1 in response to infection, we performed an RNA interference screen of C. elegans transcription factors. This screen identified zip-2, a bZIP transcription factor that is required for inducing irg-1, as well as several other genes, and is important for defense against infection by P. aeruginosa. These data indicate that zip-2 is part of a specialized pathogen response pathway that is induced by virulent strains of P. aeruginosa and provides defense against this pathogen.