Caste-specific cytochrome P450 in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Termopsidae)
Caste-specific cytochrome P450 in the damp-wood termite Hodotermopsis sjostedti (Isoptera, Termopsidae)
复制标题
DOI：
10.1111/j.1365-2583.2006.00632.x
复制
发表时间：
2006-04-01
影响因子：
2.6
通讯作者：
Miura, T
中科院分区：
文献类型：
--
作者：
Cornette, R;Koshikawa, S;Miura, T
白蚁是一种具有明确种姓制度的真社会性昆虫，这是多型性的一个例子。这种多型性是基于植物控制的差异基因表达。在湿木白蚁Hodotermopsis sjostedti，我们诱导分化成士兵种姓，通过使用保幼激素类似物处理。然后，我们研究了特定的基因表达，这出现在激素反应和触发种姓分化，使用荧光差异显示。与细胞色素P450，CYP6AM1，具有相似性的候选cDNA序列的特点，其转录显示被抑制激素治疗后1至3天之间。CYP6AM1在伪狂犬病和士兵的脂肪体中特异性表达。这P450的推定功能进行了讨论相对于种姓分化系统。
Termites are eusocial insects with a well-defined caste system, which is an example of polyphenism. This polyphenism is based on hormonally controlled differential gene expression. In the damp-wood termite Hodotermopsis sjostedti, we induced differentiation into the soldier caste by using juvenile hormone analogue treatment. We then investigated specific gene expression, which appeared during the hormonal response and triggered caste differentiation, using fluorescent differential display. A candidate cDNA sequence with similarity to cytochromes P450, CYP6AM1, was characterized and its transcript shown to be repressed between 1 and 3 days after hormone treatment. CYP6AM1 was specifically expressed in the fat body of pseudergates and soldiers. The putative function of this P450 is discussed with respect to the caste differentiation system.