Biochemical and ultrastructural studies on estrogen-induced pituitary tumors in F344 rats.
Biochemical and ultrastructural studies on estrogen-induced pituitary tumors in F344 rats.
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F344 大鼠雌激素诱导垂体肿瘤的生化和超微结构研究。
DOI：
10.1093/jnci/61.3.753
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
Carlos Libertun
中科院分区：
文献类型：
--
作者：
Alejandro F. De Nicola;Irene von Lawzewitsch;Susana E. Kaplan;Carlos Libertun
在雄性 F344 大鼠中检查了激素功能、核酸代谢以及雌激素诱导的垂体肿瘤和正常腺体的超微结构。肿瘤具有很高的催乳素（PRL）合成能力，荷瘤动物体内PRL的血浆水平升高65倍至100倍。肿瘤和正常腺体中尿苷的摄取和磷酸化为核苷酸，以及尿苷掺入总RNA的情况相似，而前一组的[3H]胸苷掺入DNA的情况是后者的两倍。 [3H]尿苷脉冲后，肿瘤和正常腺体中标记的 RNA 周转率不同。肿瘤的电子显微镜显示，产生PRL的细胞肥大、脱颗粒和增生，并在轮生中增殖。其他垂体细胞类型的数量减少。这表明螺纹结构导致了肿瘤中蛋白质和PRL的高合成率以及RNA代谢的变化。
The hormone function, the metabolism of nucleic acids, and the ultrastructure of estrogen-induced pituitary tumors and of normal glands were examined in male F344 rats. The tumors had a high capacity for prolactin (PRL) synthesis, and the plasma levels of PRL were elevated 65-fold to 100-fold in the tumor-bearing animals. Uridine uptake and phosphorylation to nucleotides, as well as uridine incorporation into total RNA, were similar in tumors and normal glands, whereas [3H]thymidine incorporation into DNA was double in the former group as compared to the latter. After a [3H]uridine pulse, labeled RNA turnover was different in tumors and normal glands. Electron microscopy of the tumors revealed hypertrophy, degranulation, and hyperplasia of cells producing PRL with proliferation of their ergastoplasm in whorls. Other pituitary cell types were reduced in number. It is suggested that the whorl configuration caused the high rate of protein and PRL synthesis as well as the changes in RNA metabolism displayed by the tumors.