Patterning of morphogenetic cell behaviors in neural ectoderm of Xenopus laevis
Patterning of morphogenetic cell behaviors in neural ectoderm of Xenopus laevis
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DOI：
10.1111/j.1749-6632.1998.tb10124.x
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Keller, RE
中科院分区：
文献类型：
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作者：
Elul, T;Koehl, MAR;Keller, RE
During vertebrate neurulation, neural ectoderm undergoes dramatic convergent extension movements, narrowing mediolaterally and lengthening anteroposteriorly. Despite the importance of convergent extension in central nervous system development, nothing is known about the patterning of morphogenetic cell behaviors in this process. Here, we examine the patterning of cell motility driving neural convergent extension in the clawed toad, Xenopus laevis. In Xenopus, neural convergent extension is induced by interactions with the neighboring mesoderm tissue. 1, 2 Recently, we showed that neural deep cell explants removed from late gastrula embryos undergo convergent extension autonomously. 1 Cells in these explants have mediolaterally biased protrusive activity, which they appear to use to intercalate themselves into a longer, narrower array. Other work from our lab has shown that persistent contact with the mesoderm induces regional differentiations in the neural ectoderm such as formation of the floorplate and columnarization of deep cells. 3 In order to determine if persistent contact with the mesoderm also modifies motility driving neural convergent extension we made high-resolution time-lapse videos of neural deep cell explants with underlying mesoderm. We observed striking modifications in the motility exhibited in neural deep cell explants without mesoderm. Notably, protrusive activity was patterned along the mediolateral axis of these explants, with lateral and medial cells exhibiting different motility behaviors. These data provide further insights into the cellular mechanism of neural convergent extension and underscore the importance of tissue context in determining morphogenetic mechanism. aThis work was funded by NIH Grant HDD25594 to Ray Keller, and an HHMI Predoctoral
