Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses
Characterization of tomato Cycling Dof Factors reveals conserved and new functions in the control of flowering time and abiotic stress responses
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DOI：
10.1093/jxb/ert451
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发表时间：
2014-03-01
影响因子：
6.9
通讯作者：
Medina, Joaquin
中科院分区：
文献类型：
--
作者：
Corrales, Alba-Rocio;Nebauer, Sergio G.;Medina, Joaquin
DNA与一个手指（DOF）转录因子的结合参与植物生长发育的多个方面，但它们在非生物胁迫耐受性中的确切作用在很大程度上是未知的。在这里，我们报告了一组五个番茄DOF基因，同源的拟南芥循环DOF因子（CDF），参与响应干旱和盐胁迫和开花时间控制的基因特异性的方式作为转录调节因子的功能。SlCDF 15是核蛋白，其显示与典型DNA靶序列的不同亲和力的特异性结合，并在体内呈现不同的转录激活能力。SlCDF15基因表现出不同的昼夜表达模式，并在响应渗透，盐，热，低温胁迫差异诱导。过表达SlCDF 1或SlCDF 3的拟南芥植物表现出增加的耐旱性和耐盐性。此外，各种胁迫响应基因，如COR15，RD29A和RD10的表达，在过表达系中被差异激活。有趣的是，在拟南芥中，过表达SlCDF 3而不是SlCDF 1通过调节开花控制基因如CO和FT的表达来促进延迟开花。总的来说，我们的数据连接SLCDF的未描述的功能相关的非生物胁迫耐受性和开花时间，通过特定的靶基因的调节和特定的代谢产物的增加。
DNA binding with One Finger (DOF) transcription factors are involved in multiple aspects of plant growth and development but their precise roles in abiotic stress tolerance are largely unknown. Here we report a group of five tomato DOF genes, homologous to Arabidopsis Cycling DOF Factors (CDFs), that function as transcriptional regulators involved in responses to drought and salt stress and flowering-time control in a gene-specific manner. SlCDF15 are nuclear proteins that display specific binding with different affinities to canonical DNA target sequences and present diverse transcriptional activation capacities in vivo. SlCDF15 genes exhibited distinct diurnal expression patterns and were differentially induced in response to osmotic, salt, heat, and low-temperature stresses. Arabidopsis plants overexpressing SlCDF1 or SlCDF3 showed increased drought and salt tolerance. In addition, the expression of various stress-responsive genes, such as COR15, RD29A, and RD10, were differentially activated in the overexpressing lines. Interestingly, overexpression in Arabidopsis of SlCDF3 but not SlCDF1 promotes late flowering through modulation of the expression of flowering control genes such as CO and FT. Overall, our data connect SlCDFs to undescribed functions related to abiotic stress tolerance and flowering time through the regulation of specific target genes and an increase in particular metabolites.