Ecophysiological characterization of Penicillium expansum population in lleida (Spain).
Ecophysiological characterization of Penicillium expansum population in lleida (Spain).
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DOI：
10.1016/j.ijfoodmicro.2007.12.014
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发表时间：
2008-03
影响因子：
5.4
通讯作者：
H. Morales;S. Marín;L. Obea;B. Patiño;M. Domenech;A. Ramos;V. Sanchis
中科院分区：
文献类型：
--
作者：
H. Morales;S. Marín;L. Obea;B. Patiño;M. Domenech;A. Ramos;V. Sanchis
扩展青霉（Penicilliumexpansum）是一种产棒曲霉素的真菌，是引起冷藏苹果和梨腐烂的最重要的真菌。这可能导致棒曲霉素污染的副产品。该测定的目的是评估导致莱利达（西班牙）收获后水果腐败的扩展青霉种群的表型和生理变异性。2004年和2005年两个季节，从腐烂的果实中总共获得了101个扩展疫霉菌株。两个季节的观测结果存在显著差异。在每个季节的菌株的变异性似乎部分解释了在培养基中的生长，棒曲霉素积累和抗真菌剂的差异。在几乎100%的菌株中检测到棒曲霉素的产生。虽然不能完全解释扩展鞭金藻种群中存在的变异性，但在某些情况下，上述变量可以解释74%的多样性。所获得的结果表明，在每个季节存在不同的种群的扩展青霉，并可能解释两个季节之间观察到的杀菌剂抗性的差异。殖民苹果肉的能力和水果殖民化的一些变量是不是一个来源的变化，无论是在每个季节，也不是两个季节进行比较。由于储藏室每个季节都要进行清洁和消毒，这表明每个季节储藏室中的种群都只从能够在苹果果肉上定居的菌株发展而来。这可能导致孢子快速形成和孢子的传播。
Penicillium expansum, a patulin producer fungus, is the most important fungus causing decay in cold stored both apples and pears. This can lead to patulin contaminated by-products. The aim of this assay was to evaluate the phenotypical and physiological variability in the population of P. expansum that cause fruit spoilage in post-harvest stages in Lleida (Spain). In total, 101 isolates of P. expansum from the 2004 and the 2005 seasons were obtained from decayed fruits. Significant differences were found in the observations from both seasons. Variability of the isolates in each season seemed to be partially explained by differences in growth in media, patulin accumulation and resistance to fungicides. Patulin production was detected in almost 100% of the isolates. Variability existing in P. expansum population could not be totally explained, but the above mentioned variables explained up to 74% of the diversity in some cases. The results obtained point to the existence of different populations of P. expansum in each season and may explain the differences in fungicide resistance observed between both seasons. The capacity to colonize apple flesh and some variables involved in fruit colonization were not a source of variation neither in each season nor when both seasons were compared. As storage rooms are cleaned and disinfected each season, this suggests that each season, the populations in storage rooms develop only from strains capable to colonize apple flesh. This may lead to rapid sporulation and spreading of spores.