Enrichment of steroid hormones in water with porous and hydrophobic polymer-based SPE followed by HPLC-UV determination.
Enrichment of steroid hormones in water with porous and hydrophobic polymer-based SPE followed by HPLC-UV determination.
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DOI：
10.1002/jssc.201300663
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发表时间：
2013-10
影响因子：
3.1
通讯作者：
Yinfen Hu;Man-man Zhang;C. Tong;Jianmin Wu;Wei-ping Liu
中科院分区：
文献类型：
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作者：
Yinfen Hu;Man-man Zhang;C. Tong;Jianmin Wu;Wei-ping Liu
人们对地表水中类固醇激素的持久性非常关注。由于这些化合物在水样中的浓度通常处于痕量水平，因此有效富集类固醇激素对于进一步分析至关重要。在这项工作中，多孔和疏水聚合物的合成和表征。在合成过程中用作致孔剂的溶剂的组合物被证明对聚合物的形态有影响，该聚合物被成功地用作SPE吸附剂，用于同时富集地表水样品中的类固醇激素。类固醇激素在定制聚合物上的回收率范围为93.4%至106.2%，而在商业化ENVI-18、LC-18和Oasis HLB上的回收率范围分别为54.8%至104.9%、66%至93.6%和77.2%至106%。采用自制的吸附剂对5种甾体激素进行富集，用HPLC-UV同时测定。在此基础上，建立了固相萃取-高效液相色谱法。该方法对雌三醇、雌二醇、雌酮、雄烯二酮和孕酮的检出限分别为0.07、0.43、0.61、0.27和0.42 μg/L，精密度和重现性RSD分别为<6.40和7.49%。
There have been great concerns about the persistence of steroid hormones in surface water. Since the concentrations of these compounds in water samples are usually at a trace level, the efficient enrichment of steroid hormones is vital for further analysis. In this work, a porous and hydrophobic polymer was synthesized and characterized. The composition of solvent used as porogen in the synthetic process was shown to have an effect on the morphology of the polymer, which was successfully used as an SPE sorbent for simultaneously enriching steroid hormones in surface water samples. The recoveries of the steroid hormones on the custom-made polymer ranged from 93.4 to 106.2%, whereas those on commercialized ENVI-18, LC-18, and Oasis HLB ranged from 54.8 to 104.9, 66 to 93.6, and 77.2 to 106%, respectively. Five types of steroid hormones were simultaneously measured using HPLC-UV after they were enriched by the custom-made sorbent. Based on these findings, the SPE-HPLC method was developed. The LODs of this method for estriol, estradiol, estrone, androstenedione, progesterone were 0.07, 0.43, 0.61, 0.27, and 0.42 μg/L, respectively, while precision and reproducibility RSDs were <6.40 and 7.49%, respectively.