Effect of Multiple Inhibitions in Corncob Hydrolysate on the Lipid Production by Rhodotorula glutinis
Effect of Multiple Inhibitions in Corncob Hydrolysate on the Lipid Production by Rhodotorula glutinis
复制标题
玉米芯水解物多重抑制对粘红酵母产脂的影响
DOI：
10.1021/acs.energyfuels.7b02159
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发表时间：
2017-11-01
期刊：
影响因子：
5.3
通讯作者：
Zhang, Jianan
中科院分区：
文献类型：
--
作者：
Lin, Zhangnan;Zhou, Yujie;Zhang, Jianan
预处理过程中产生的木质纤维生物质降解副产物对微生物有多种负面影响。以粘红酵母为研究对象，研究了多种抑制剂对粘红酵母细胞生长和产脂的影响。采用单因素实验研究各单项抑制作用。在三种抑制剂中，对细胞生长毒性最大的是呋喃西林，其次是羟甲基呋喃，然后是醋酸。在0.8g/L浓度下，生物量和脂肪含量分别比对照降低了18.1%和27.0%。粘性葡萄球菌对醋酸有很强的耐受性。当醋酸浓度低于10g/L时，甚至可以刺激生物量和脂肪的产生。利用响应面方法(RSM)进一步研究了多重抑制对细胞生长和脂肪产生的影响。甲醛与醋酸的相互作用显著(p<；0.0001)，但对乙酸乙酯的影响较小。
Lignocellulosic biomass degradation byproducts generated in the pretreatment process have various negative impacts on microbes. This work investigated the effects of multiple inhibitors, such as acetic acid, furfural, and 5-hydroxymethylfurfural (HMF), on cell growth and lipid production by Rhodotorula glutinis. Single-factor experiments were performed to study the individual inhibitory actions. Among the three inhibitors, furfural is the most toxic for the cell growth, followed by HMF and then acetic acid. At 0.8 g/L furfural, the biomass and lipid concentrations decreased by 18.1 and 27.0% compared to the control. R. glutinis has a strong tolerance to acetic acid. Biomass and lipid production could even be stimulated when the acetic acid concentration was lower than 10 g/L. The effect of multiple inhibitions on the cell growth and lipid production was further studied using response surface methodology (RSM). The interaction of furfural and acetic acid was significant (p < 0.0001), but for the case of ac...