Identification of shark species in commercial products using DNA barcoding
Identification of shark species in commercial products using DNA barcoding
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DOI：
10.1016/j.fishres.2018.10.010
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发表时间：
2019-02-01
影响因子：
2.4
通讯作者：
Hernandez, Eduardo L.
中科院分区：
文献类型：
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作者：
Hellberg, Rosalee S.;Isaacs, Rachel B.;Hernandez, Eduardo L.
鲨鱼在全球范围内收获，并以各种商业产品出售。然而，它们特别容易受到过度捕捞的影响，许多物种被认为是受保护或濒危的。本研究的目的是鉴定不同商业鲨鱼产品中的物种，并评估三种不同的DNA条码引物集的有效性。这项研究收集了35种产品，包括鱼片、肉干、汤和软骨丸。使用细胞色素C氧化酶亚单位(COI)基因内的两组全长和一组小型条形码引物对这些产物进行DNA条形码编码。然后，使用序列数据库和基于特征的分析，对成功测序的样本进行物种水平的分析和鉴定。综合三对引物的结果，74.3%的扩增产物达到了种水平。迷你条形码对物种识别的成功率最高(54.3%)，并且具有广泛的识别能力。在26种被确认的产品中，有6种被发现标签错误或可能标签错误，包括鲨鱼软骨丸、鲨鱼干和鱼翅汤的样品。根据国际自然保护联盟(IUCN)的红色名录，6种产品含有《濒危野生动植物种国际贸易公约》(CITES)附录中所列的物种，23种产品含有濒临威胁、脆弱或濒危物种。这项研究显示，DNA条码引物组合可用于识别各种加工鲨鱼产品中的物种，从而帮助保护和监测工作。
Sharks are harvested globally and sold in a variety of commercial products. However, they are particularly vulnerable to overfishing and many species are considered protected or endangered. The objective of this study was to identify species in various commercial shark products and to assess the effectiveness of three different DNA barcoding primer sets. Thirty-five products were collected for this study, including fillets, jerky, soup, and cartilage pills. DNA barcoding of these products was undertaken using two full-length primer sets and one mini-barcode primer set within the cytochrome c oxidase subunit (COI) gene. Successfully sequenced samples were then analyzed and identified to the species level using sequence databases and character-based analysis. When the results of all three primer sets were combined, 74.3% of the products were identified to the species level. Mini-barcoding showed the highest success rate for species identification (54.3%) and allowed for a wide range of identification capability. Six of the 26 identified products were found to be mislabeled or potentially mislabeled, including samples of shark cartilage pills, shark jerky, and shark fin soup. Six products contained species listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendices and 23 products contained near-threatened, vulnerable or endangered species according to the International Union for the Conservation of Nature (IUCN) Red List Overall, this study revealed that a combination of DNA barcoding primers can be utilized to identify species in a variety of processed shark products and thereby assist with conservation and monitoring efforts.