Isolation of Crb1, a mouse homologue of Drosophila crumbs, and analysis of its expression pattern in eye and brain
Isolation of Crb1, a mouse homologue of Drosophila crumbs, and analysis of its expression pattern in eye and brain
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DOI：
10.1016/s0925-4773(01)00568-8
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发表时间：
2002-01-01
影响因子：
2.6
通讯作者：
Broccoli, V
中科院分区：
文献类型：
--
作者：
den Hollander, AI;Ghiani, M;Broccoli, V
人类Crumbs同源物1（CRB 1）基因的突变导致严重的视网膜营养不良。CRB1与果蝇Crumbs同源，Crumbs是建立和维持上皮极性所必需的蛋白质。我们已经分离出小鼠直系同源基因Crb1，并分析了其在胚胎和出生后阶段的表达模式。Crb1只在眼睛和中枢神经系统中表达。在发育中的眼睛中，在视网膜祖细胞中检测到Crb1的表达，并且随后变得限于分化的感光细胞，在那里它保持活性直到成人阶段。在发育中的神经管中，Crb1的表达仅限于其最腹侧的结构，与Nkx2.2的表达结构域相一致。在成人大脑中，Crb1表达被定义为在成年期发生神经元产生和迁移的区域。(C)2002爱思唯尔科学爱尔兰有限公司保留所有权利。
Mutations in the human Crumbs homologue 1 (CRB 1) gene cause severe retinal dystrophies. CRB1 is homologous to Drosophila Crumbs, a protein essential for establishing and maintaining epithelial polarity. We have isolated the mouse orthologue, Crb1, and analyzed its expression pattern in embryonic and post-natal stages. Crb1 is expressed exclusively in the eye, and the central nervous system. In the developing eye, expression of Crb1 is detected in the retinal progenitors, and later on becomes restricted to the differentiated photoreceptor cells where it remains active up to the adult stage. In the developing neural tube, expression of Crb1 is restricted to its most ventral structures, coinciding with the expression domain of Nkx2.2. In the adult brain, Crb1 expression is defined to areas where the production and migration of neurons occurs in adulthood. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.