Proteomic analysis reveals Warburg effect and anomalous metabolism of glutamine in pancreatic cancer cells.
Proteomic analysis reveals Warburg effect and anomalous metabolism of glutamine in pancreatic cancer cells.
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蛋白质组学分析揭示了胰腺癌细胞中的瓦尔堡效应和谷氨酰胺代谢异常。
DOI：
10.1021/pr2009274
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发表时间：
2012-02-03
影响因子：
4.4
通讯作者：
Petricoin EF
中科院分区：
文献类型：
--
作者：
Zhou W;Capello M;Fredolini C;Racanicchi L;Piemonti L;Liotta LA;Novelli F;Petricoin EF
在本工作中，我们利用LTQ-Orbitrap对胰腺导管腺癌(PDAC)细胞系PANC-1和正常胰腺管细胞的蛋白质组进行了LTQ-Orbitrap质谱分析，并从每个样本中鉴定了1700多个蛋白质组。在光谱计数无标记定量方法的基础上，我们鉴定了大量差异表达的代谢酶和蛋白质，涉及细胞骨架、细胞黏附、转运、转录、翻译和细胞增殖。数据表明，PANC-1的代谢途径发生了改变，这与Warburg效应一致。此外，比较MS分析揭示了谷氨酰胺的异常代谢，表明谷氨酰胺在PANC-1的核苷酸和氨基酸生物合成中作为氮供体被大量消耗。我们的分析提供了潜在的关于PANC-1代谢的全面图景，这可能作为PDAC新的诊断和治疗的基础。
In this present work, we characterized the proteomes of pancreatic ductal adenocarcinoma (PDAC) cell line PANC-1 and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap and identified more than 1700 proteins from each sample. On the basis of the spectra count label-free quantification approach, we identified a large number of differentially expressed metabolic enzymes and proteins involved in cytoskeleton, cell adhesion, transport, transcription, translation, and cell proliferation as well. The data demonstrated that metabolic pathways were altered in PANC-1, consistent with the Warburg effect. In addition, the comparative MS analysis unveiled anomalous metabolism of glutamine, suggesting that glutamine was largely consumed as a nitrogen donor in nucleotide and amino acid biosynthesis in PANC-1. Our analysis provides a potentially comprehensive picture of metabolism in PANC-1, which may serve as the basis of new diagnostics and treatment of PDAC.
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影响因子：
56.9
作者：
Jaakkola, P;Mole, DR;Ratcliffe, PJ
通讯作者：
Ratcliffe, PJ
影响因子：
--
作者：
Erickson JW;Cerione RA
通讯作者：
Cerione RA
影响因子：
7
作者：
Ishihama, Y;Oda, Y;Mann, M
通讯作者：
Mann, M
DOI：
10.1073/pnas.93.23.12969
发表时间：
1996-11-12
影响因子：
11.1
作者：
Arany, Z;Huang, LE;Livingston, DM
通讯作者：
Livingston, DM
影响因子：
11.4
作者：
CERINI, C;KERJAN, P;SEMERIVA, M
通讯作者：
SEMERIVA, M