Thermal analysis reveals differential effects of various crosslinkers on bovine annulus fibrosis.
Thermal analysis reveals differential effects of various crosslinkers on bovine annulus fibrosis.
复制标题
热分析揭示了各种交联剂对牛纤维环纤维化的不同影响。
DOI：
10.1002/jor.21189
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Hedman,Tom
中科院分区：
文献类型：
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作者：
Zhu,Keng;Slusarewicz,Paul;Hedman,Tom
在体内注射蛋白交联试剂恢复椎间盘的力学性能是治疗退行性椎间盘疾病的一种新方法。在这项研究中，用差示扫描量热法测量了六种不同交联剂处理后的胶原在椎间盘环中的热稳定性。所使用的交联剂有；L‐threose (LT), genipin (GP), methylglyoxal (MG), 1‐ethyl‐3‐（3‐二甲氨基丙基）carbodi亚胺hydrochloride (EDC), glutaraldehyde （GA）和proanthrocyanidin （PA）。未经处理的组织在约66-68°C时显示出明显的峰值。未处理和交联剂处理的圆盘环组织样品的内热模式的比较表明，在处理后的更高温度下出现了一个新的峰值。新峰的温度定性地依赖于交联剂的顺序GA > MG > GP > PA = EDC > LT，表明环状组织中胶原热稳定性的增强与交联剂的性质有关。此外，处理过和未处理过的组织中较低温度（非交联）峰的焓比，以及处理过的组织中较高和较低温度峰的焓比，都表明不同的试剂以不同的效率交联组织。我们的数据表明，GP穿透椎间盘并形成长距离和短程交联的能力可能使其成为最适合临床开发的候选材料。此外，长距离和短程交联剂的二元组合，如PA与LT，由于其物理化学性质的本质不同，也可能提供协同效应。©2010骨科研究学会。Wiley期刊公司出版。[J]中华骨科杂志，2011
Treatment of a pathological spinal disc in vivo by injection of protein crosslinking reagents to restore the disc's mechanical properties is a new approach to the treatment of degenerative disc disease. In this study, the thermal stability of the collagen in disc annulus was measured by differential scanning calorimetry following treatment with six different crosslinking agents. The crosslinkers used were; L‐threose (LT), genipin (GP), methylglyoxal (MG), 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC), glutaraldehyde (GA), and proanthrocyanidin (PA). Untreated tissue displayed a prominent peak at about 66–68°C. Comparison of endothermal patterns of untreated and crosslinker‐treated disc annulus tissue samples showed that a new peak appeared at a higher temperature following treatment. The temperature of the new peak qualitatively depended on the crosslinker in the following order GA > MG > GP > PA = EDC > LT, suggesting that the enhanced thermal stability of collagen in the annulus tissue was related to the nature of the crosslinker. Also, the enthalpic ratios of the lower temperature (noncrosslinked) peaks in the treated and untreated tissue, and of the higher and lower temperature peaks in the treated tissue, both indicated that the various agents crosslinked the tissue with different efficiencies. Our data suggest that the ability of GP to penetrate into the disc and form long‐ and short‐range crosslinks may make it the most suitable candidate for clinical development. In addition, binary combinations of long‐ and short‐range crosslinkers, such as PA with LT, may also provide synergistic effects due to their substantially different physicochemical properties. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:8–13, 2011