Modified GM3 gangliosides produced by metabolic oligosaccharide engineering
Modified GM3 gangliosides produced by metabolic oligosaccharide engineering
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DOI：
10.1016/j.bmcl.2011.04.128
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Kohler, Jennifer J.
中科院分区：
文献类型：
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作者：
Whitman, Chad M.;Yang, Fan;Kohler, Jennifer J.
代谢寡糖工程是改变细胞唾液酸苷结构的有效途径。这种方法依赖于用N-乙酰甘露糖胺(ManNAc)类似物培养细胞，这些细胞被代谢成唾液酸类似物，并添加到糖蛋白和糖脂中。在这里，我们使用了两个缺乏ManNAc生物合成的细胞系，并检测了它们代谢一组唾液酸甘露糖苷的ManNAc类似物的相对能力。除了测量全球唾液酸苷的产量外，我们还研究了含唾液酸的糖脂GM3的生物合成。我们发现，这两个细胞系在区分不同形式的ManNAc的能力上存在差异。此外，我们的数据表明，修饰形式的唾液酸可能优先被结合到某些唾液酸苷中，而被排除在其他唾液酸苷之外。综上所述，我们的结果表明，对唾液酸苷生产的全球分析可能会掩盖唾液酸苷特定的差异。这些发现对包括成像和蛋白质组学在内的代谢寡糖工程的下游应用具有重要意义。(C)2011爱思唯尔有限公司。保留所有权利。
Metabolic oligosaccharide engineering is powerful approach to altering the structure of cellular sialosides. This method relies on culturing cells with N-acetylmannosamine (ManNAc) analogs that are metabolized to their sialic acid counterparts and added to glycoproteins and glycolipids. Here we employed two cell lines that are deficient in ManNAc biosynthesis and examined their relative abilities to metabolize a panel of ManNAc analogs to sialosides. In addition to measuring global sialoside production, we also examined biosynthesis of the sialic acid-containing glycolipid, GM3. We discovered that the two cell lines differ in their ability to discriminate among the variant forms of ManNAc. Further, our data suggest that modified forms of sialic acid may be preferentially incorporated into certain sialosides and excluded from others. Taken together, our results demonstrate that global analysis of sialoside production can obscure sialoside-specific differences. These findings have implications for downstream applications of metabolic oligosaccharide engineering, including imaging and proteomics. (C) 2011 Elsevier Ltd. All rights reserved.