Styryl-based wavelength-ratiometric probes: a new class of fluorescent calcium probes with long wavelength emission and a large Stokes' shift.
Styryl-based wavelength-ratiometric probes: a new class of fluorescent calcium probes with long wavelength emission and a large Stokes' shift.
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DOI：
10.1006/abio.1993.1422
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发表时间：
1993-09
影响因子：
2.9
通讯作者：
E. Akkaya;J. Lakowicz
中科院分区：
文献类型：
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作者：
E. Akkaya;J. Lakowicz
我们描述了一种含有钙离子螯合基团BAPTA的苯并噻唑探针的化学合成和荧光光谱表征。该探针的可见吸收光谱与钙离子络合后，其最大吸收波长从508 nm急剧移动到407 nm，钙离子解离常数为1.5微米。以615 nm为中心的发射极大值与吸收峰有很好的偏移。结合Ca~(2+)后，发射光谱呈现小的蓝移，这使得该探针有可能被用作单激发波长的发射波长比率探针。该探针很可能是一系列长波长比率钙离子探针中的第一个，其结构可以被修改以提高量子产率或改变钙离子亲和力。
We describe the chemical synthesis and fluorescence spectral characterization of a styryl-benzothiazole probe which contains the Ca2+ chelating group BAPTA as an integral part of the chromophoric system. The visible absorption spectrum of this probe displays a dramatic shift in the long wavelength maxima from 508 to 407 nm upon complexation with Ca2+, with a Ca2+ dissociation constant of 1.5 microM. The emission maximum centered at 615 nm is well shifted from the absorption. The emission spectrum displays a small blue shift upon binding Ca2+, allowing this probe to possibly be used as an emission wavelength-ratiometric probe using a single-excitation wavelength. This probe is likely to be the first of a series of long-wavelength ratiometric Ca2+ probes whose structure can be modified for improved quantum yield or altered Ca2+ affinity.