Co-infection of white-tailed deer with multiple strains of Ehrlichia chaffeensis.
Co-infection of white-tailed deer with multiple strains of Ehrlichia chaffeensis.
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白尾鹿与多种查菲埃里希体的共同感染。
DOI：
10.1089/vbz.2006.6.140
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Little,SusanE
中科院分区：
文献类型：
--
作者：
Varela-Stokes,AndreaS;Stokes,JohnV;Davidson,WilliamR;Little,SusanE
我们研究了不同链节长度的查菲埃立克体菌株对鹿的影响，这些菌株在可变长度聚合酶链式反应靶基因(VLPT)和120 kDa抗原基因上具有不同的串联重复数。我们假设感染一种菌株会对感染其他菌株产生免疫力。所有最初接触A株(604-2)的鹿，在感染后10天(DPI)，所有的鹿都变成了PCR和培养阳性。用A株感染后再接种B株(623-4)的3只鹿感染了B株，2头感染A株后再接种C株(125B)的鹿感染了C株，其中3只鹿感染了B株后再接种C株，其中1只对C株呈聚合酶链式反应阳性。一项互补的体外研究表明，暴露于两个VLPT重复序列不同的菌株可能会导致DH82细胞的混合感染。这些结果补充了之前的一项研究，进一步表明鹿可以连续感染多达三种E菌株。查菲斯。这表明，在边缘无浆体等相关生物中描述的一种现象--竞争性排斥，即感染一种菌株后不会感染同一物种的另一种不同的菌株--可能不会发生。查菲斯。
We investigated the effect of exposing deer to multiple strains ofEhrlichia chaffeensisthat differed in number of tandem repeats in either the variable-length PCR target (VLPT) gene or 120 kDa antigen gene. We hypothesized that infection with one strain would provide immunity to infection with other strains ofE. chaffeensis.All deer initially exposed to strain A (604-2) became PCR and culture positive by 10 days post-infection (DPI). Three deer infected with strain A and subsequently inoculated with strain B (623-4) became infected with strain B. Two deer infected with strain A and subsequently inoculated with strain C (125B) became infected with strain C. Of three deer, each infected with strain B and subsequently inoculated with strain C, one was PCR positive for strain C. Of three deer previously inoculated with both strains A and B, and subsequently inoculated with strain C, one showed delayed evidence of strain C. Western blot analysis demonstrated that deer sera reacted differently to antigens from each exposed strain. A complementaryin vitrostudy demonstrated that exposure to two strains differing in VLPT repeats may lead to co-infection of DH82 cells. These results complement a previous study and further show that deer can become sequentially infected with up to three strains ofE. chaffeensis. This suggests that competitive exclusion, a phenomenon described in related organisms such asAnaplasma marginalewhereby infection with one strain precludes subsequent infection by a second, distinct strain of the same species, may not occur withE. chaffeensis.