Tractography-Pathology Correlations in Traumatic Brain Injury: A TRACK-TBI Study.
Tractography-Pathology Correlations in Traumatic Brain Injury: A TRACK-TBI Study.
复制标题
创伤性脑损伤中的纤维束成像-病理学相关性：TRACK-TBI 研究。
DOI：
10.1089/neu.2020.7373
复制
发表时间：
2021
影响因子：
4.2
通讯作者：
Keene,
中科院分区：
文献类型：
--
作者：
Nolan,AmberL;Petersen,Cathrine;Iacono,Diego;MacDonald,ChristineL;Mukherjee,Pratik;vanderKouwe,Andre;Jain,Sonia;Stevens,Allison;Diamond,BramR;Wang,Ruopeng;Markowitz,AmyJ;Fischl,Bruce;Perl,DanielP;Manley,GeoffreyT;Keene,
磁共振扩散束成像(MRI)可以推断创伤性脑损伤(TBI)患者网络连通性的变化，但由于缺乏高分辨率成像和组织病理学验证，分离的纤维束的病理基础尚未得到很好的界定。我们开发了AneX vivoMRI方案来分析750-μm各向同性分辨率的束终止，然后对脑白质病理进行组织病理学评估，并将这些方法应用于一名60岁的男子，他在颅脑损伤后26天死亡。对74个大脑半球白质区域的分析显示出脑束中断的异质性分布。相关的组织病理学发现，可变性白质损伤伴有淀粉样前体蛋白(APP)斑块沉积、神经丝阳性轴突丢失、髓鞘溶解、星形胶质细胞增多、小胶质细胞增多和血管周围含铁血黄素的巨噬细胞。多元线性回归显示神经束断裂与APP阳性轴突肿胀和神经丝丢失的密度密切相关。体外活体扩散磁共振成像可以检测到反映轴突损伤的人脑中的束断裂。
Diffusion tractography magnetic resonance imaging (MRI) can infer changes in network connectivity in patients with traumatic brain injury (TBI), but the pathological substrates of disconnected tracts have not been well defined because of a lack of high-resolution imaging with histopathological validation. We developed anex vivoMRI protocol to analyze tract terminations at 750-μm isotropic resolution, followed by histopathological evaluation of white matter pathology, and applied these methods to a 60-year-old man who died 26 days after TBI. Analysis of 74 cerebral hemispheric white matter regions revealed a heterogeneous distribution of tract disruptions. Associated histopathology identified variable white matter injury with patchy deposition of amyloid precursor protein (APP), loss of neurofilament-positive axonal processes, myelin dissolution, astrogliosis, microgliosis, and perivascular hemosiderin-laden macrophages. Multiple linear regression revealed that tract disruption strongly correlated with the density of APP-positive axonal swellings and neurofilament loss.Ex vivodiffusion MRI can detect tract disruptions in the human brain that reflect axonal injury.