Surface characterization and cytocompatibility evalution of silanized magnesium alloy AZ91 for biomedical applications
Surface characterization and cytocompatibility evalution of silanized magnesium alloy AZ91 for biomedical applications
复制标题
生物医学应用硅烷化镁合金AZ91的表面表征和细胞相容性评估
DOI：
10.1088/1468-6996/13/6/064214
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发表时间：
2012
影响因子：
5.5
通讯作者：
W.Swieszkowski
中科院分区：
文献类型：
--
作者：
A.Witecka;A.Yamamoto;H.Dybiec;W.Swieszkowski
高Al含量的镁合金在水环境中具有上级耐腐蚀性，但与纯镁相比，其细胞相容性较差。我们使用五种不同的硅烷对铸造AZ 91合金进行硅烷化以改善其细胞相容性：乙基三乙氧基硅烷（S1）、3-氨丙基三乙氧基硅烷（S2）、3-异氰酸酯基三乙氧基硅烷（S3）、苯基三乙氧基硅烷（S4）和十八烷基三乙氧基硅烷（S5）。通过水接触角测量来评估表面亲水性/疏水性。X射线光电子分析进行了调查的表面状态和化学组成的变化。所有硅烷试剂都增加了白蛋白对改性表面的吸附。体外细胞相容性评价表明，硅烷化提高了细胞在硅烷S1修饰的AZ 91上的生长。在细胞培养过程中释放的Mg 2+离子的浓度的测量表明，硅烷化不影响底物降解。
Mg alloys with high Al contents have superior corrosion resistance in aqueous environments, but poor cytocompatibility compared to that of pure Mg. We have silanized the cast AZ91 alloy to improve its cytocompatibility using five different silanes: ethyltriethoxysilane (S1), 3-aminopropyltriethoxysilane (S2), 3-isocyanatopyltriethoxysilane (S3), phenyltriethoxysilane (S4) and octadecyltriethoxysilane (S5). The surface hydrophilicity/hydrophobicity was evaluated by water contact angle measurements. X-ray photoelectron analysis was performed to investigate the changes in surface states and chemical composition. All silane reagents increased adsorption of the albumin to the modified surface. In vitro cytocompatibility evaluation revealed that silanization improved cell growth on AZ91 modified by silane S1. Measurement of the concentration of Mg 2+ ions released during the cell culture indicated that silanization does not affect substrate degradation.