PHAGOCYTOSIS - FLOW CYTOMETRIC QUANTITATION WITH FLUORESCENT MICROSPHERES
PHAGOCYTOSIS - FLOW CYTOMETRIC QUANTITATION WITH FLUORESCENT MICROSPHERES
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DOI：
10.1126/science.7053559
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发表时间：
1982-01-01
期刊：
影响因子：
56.9
通讯作者：
STEWART, CC
中科院分区：
文献类型：
--
作者：
STEINKAMP, JA;WILSON, JS;STEWART, CC
用流式细胞术分析大鼠肺巨噬细胞对均匀荧光胶乳颗粒的吞噬作用。根据细胞大小和荧光直方图确定吞噬巨噬细胞的百分比和每个细胞的颗粒数量。体内和体外吞噬数据的比较表明，吞噬灌洗巨噬细胞的百分比反映了滴注颗粒的可用性。以叠氮钠为吞噬抑制模型，可同时测定吞噬细胞百分率和细胞内颗粒数。
The phagocytosis of uniform fluorescent latex particles by pulmonary macrophages in the rat was analyzed by flow cytometric methods. The percentage of phagocytic macrophages and the number of particles per cell were determined from cell-size and fluorescence histograms. A comparison of in vivo and in vitro phagocytosis data showed that the percentage of phagocytic lavaged macrophages reflected the availability of instilled particles. With sodium azide used to model phagocytosis inhibition, it was shown that the percentage of phagocytic cells and the number of particles per cell can be determined simultaneously.