Cardiovascular tissues contain independent circadian clocks
Cardiovascular tissues contain independent circadian clocks
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DOI：
10.1081/ceh-200048933
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发表时间：
2005-02-01
影响因子：
12.3
通讯作者：
Menaker, M
中科院分区：
文献类型：
--
作者：
Davidson, AJ;London, B;Menaker, M
急性心血管事件的发生频率具有昼夜节律。这些现象背后的机制尚未完全了解，但它们可能是由于心血管系统固有的节律性。我们已经开始在大鼠心血管系统的时钟基因mPer 1的节律性的特点。mPer 1基因启动子驱动的荧光素酶活性在转基因Per 1-luc大鼠的心脏组织外植体和各种静脉和动脉培养的体外是有节奏的。在体外，在阻尼之前，组织显示出3至12个昼夜节律周期的基因表达。尽管峰值脉冲驱动的生物发光在心脏和所有动脉采样的深夜期间一致地发生，但是静脉中的节律的相位根据解剖位置而显著变化。改变相对于供体动物的光：暗周期的培养程序的时间显示，与其他一些大鼠组织，如肝脏，动脉，静脉和心脏外植体的体外节律的阶段受培养时间的影响。然而，组织之间的相位关系是一致的文化时间，这表明心血管系统的组成部分之间的昼夜节律调节的多样性。
Acute cardiovascular events exhibit a circadian rhythm in the frequency of occurrence. The mechanisms underlying these phenomena are not yet fully understood, but they may be due to rhythmicity inherent in the cardiovascular system. We have begun to characterize rhythmicity of the clock gene mPer1 in the rat cardiovascular system. Luciferase activity driven by the mPer1 gene promoter is rhythmic in vitro in heart tissue explants and a wide variety of veins and arteries cultured from the transgenic Per1-luc rat. The tissues showed between 3 and 12 circadian cycles of gene expression in vitro before damping. Whereas peak per]driven bioluminescence consistently occurred during the late night in the heart and all arteries sampled, the phases of the rhythms in veins varied significantly by anatomical location. Varying the time of the culture procedure relative to the donor animal's light:dark cycle revealed that, unlike some other rat tissues such as liver, the phases of in vitro rhythms of arteries, veins, and heart explants were affected by culture time. However, phase relationships among tissues were consistent across culture times; this suggests diversity in circadian regulation among components of the cardiovascular system.