Global gene expression of cells attached to a tissue engineering scaffold
Global gene expression of cells attached to a tissue engineering scaffold
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DOI：
10.1016/j.biomaterials.2004.01.025
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发表时间：
2004-11-01
期刊：
影响因子：
14
通讯作者：
Bertozzi, CR
中科院分区：
文献类型：
--
作者：
Klapperich, CM;Bertozzi, CR
组织工程的目标是产生一种支架材料，该支架材料将引导细胞分化并在损伤部位再生功能性替代组织。关于细胞如何在分子水平上对组织工程支架材料做出反应，我们知之甚少。在这项工作中，我们使用寡核苷酸微阵列询问与细胞生物材料相互作用相关的基因表达谱。我们种植胶原-糖胺聚糖网，一种广泛使用的组织工程支架材料。与人IMR-90成纤维细胞进行比较，并将转录水平与在组织培养聚苯乙烯上生长的对照细胞进行比较。参与细胞信号传导、细胞外基质重塑、炎症、血管生成和缺氧的基因都在胶原-GAG网格上的细胞中被激活。了解支架对附着细胞的影响将有助于设计改进的组织工程材料。(C)2004爱思唯尔有限公司保留所有权利。
A goal of tissue engineering is to produce a scaffold material that will guide cells to differentiate and regenerate functional replacement tissue at the site Of injury. Little is known about how cells respond on a molecular level to tissue engineering scaffold materials. In this work we used oligonucleotide microarrays to interrogate gene expression profiles associated with cell-biomaterial interactions. We seeded collagen-glycosaminoglycan meshes, a widely used tissue engineering scaffold material. with human IMR-90 fibroblasts and compared transcript levels with control cells grown on tissue Culture polystyrene. Genes involved in cell signaling, extracellular matrix remodeling, inflammation, angiogenesis and hypoxia were all activated in cells on the collagen-GAG mesh. Understanding the impact of a scaffold on attached cells will facilitate the design of improved tissue engineering materials. (C) 2004 Elsevier Ltd. All rights reserved.