Quantitative determination of the intracellular concentration of the various forms of HPr, a phosphocarrier protein of the phosphoenolpyruvate: sugar phosphotransferase system in growing cells of oral streptococci.
Quantitative determination of the intracellular concentration of the various forms of HPr, a phosphocarrier protein of the phosphoenolpyruvate: sugar phosphotransferase system in growing cells of oral streptococci.
复制标题
定量测定口腔链球菌生长细胞中各种形式 HPr 的细胞内浓度，HPr 是磷酸烯醇丙酮酸的磷酸载体蛋白：糖磷酸转移酶系统。
DOI：
10.1016/0003-2697(91)90112-7
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发表时间：
1991
影响因子：
2.9
通讯作者：
Reizer,J
中科院分区：
文献类型：
--
作者：
Vadeboncoeur,C;Brochu,D;Reizer,J
一个简单的程序定量估计不同磷酸化形式的磷酸化载体蛋白HPr在口腔链球菌生长细胞中的描述。通过酸化培养基并同时加入离子载体短杆菌肽D，细胞的生长迅速停止。该程序使酶I、HPr（Ser）激酶、HPr（Ser-P）磷酸酶和参与别构效应物代谢的酶以及HPr磷酸化的底物失活。然后通过交叉免疫电泳测定细胞内HPr（His His-P）、HPr（Ser-P）、HPr（His His-P）（Ser-P）和游离HPr的浓度。
A simple procedure for quantitative estimation of the different phosphorylated forms of the phosphocarrier protein HPr in growing cells of oral streptococci is described. The growth of the cells was rapidly stopped by acidification of the medium and concomitant addition of the ionophore Gramicidin D. This procedure inactivated Enzyme I, HPr(Ser) kinase, HPr(Ser-P) phosphatase, and the enzymes involved in the metabolism of the allosteric effectors as well as the substrates of HPr phosphorylation. The cellular concentrations of HPr(His∼P), HPr(Ser-P), HPr(His∼P)(Ser-P), and free HPr were then determined by crossed immunoelectrophoresis.