Identification and mass spectrometric characterization of glycosylated flavonoids in Triticum durum plants by high-performance liquid chromatography with tandem mass spectrometry
Identification and mass spectrometric characterization of glycosylated flavonoids in Triticum durum plants by high-performance liquid chromatography with tandem mass spectrometry
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DOI：
10.1002/rcm.2185
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发表时间：
2005-01-01
影响因子：
2
通讯作者：
Laganà, A
中科院分区：
文献类型：
--
作者：
Cavaliere, C;Foglia, P;Laganà, A
建立了硬粒小麦幼茎和幼叶中黄酮苷类化合物的质谱检测和半定量分析方法。将约100 g样品冻干并研磨，然后使用高效液相色谱法（HPLC）提取、净化和分馏感兴趣的化合物。使用四极杆线性离子阱仪器进行串联质谱分析，该仪器具有信息依赖性数据采集（IDA）方案，该方案循环两个实验，增强的MS扫描和增强的产物离子扫描。各种糖缀合物，这是所有的衍生物，只有四个黄酮，芹菜素，毛地黄黄酮，chrysoeriol和麦黄酮，被确定，属于以下类别：7个单糖苷，31个二糖苷，15个三糖苷和1个四糖苷。其中发现了一些酰化糖苷，麦黄酮衍生物仅以O-糖苷存在，而芹菜素和毛地黄黄酮总是以C-糖苷存在。以槲皮素单苷和双苷为内标物进行半定量测定。版权所有（C）2005约翰威利父子有限公司
A mass spectrometric method for extensive detection and semi-quantitative determination of flavonoid glycosides in stem and leaves of young Triticum durum plants is presented. About 100 g of sample were Iyophilized and ground, and the compounds of interest were then extracted, cleaned-up, and fractionated using high-performance liquid chromatography (HPLC). Tandem mass spectrometry analyses were performed using a quadrupole-linear ion trap instrument with an information-dependent data acquisition (IDA) protocol that looped two experiments, enhanced MS scan and enhanced product ion scan. Various glycoconjugates, which are all derivatives of only four flavones, apigenin, luteolin, chrysoeriol and tricin, were identified and belong to the following categories: 7 monoglycosides, 31 diglycosides, 15 triglycosides and 1 tetraglycoside. Among these some acylated glycosides were found. Tricin derivatives are present exclusively as O-glycosides, while apigenin and luteolin are present always as C-glycosides. Semi-quantitative estimation was performed by using the monoglycoside and diglycoside of quercetin as internal standards. Copyright (C) 2005 John Wiley & Sons, Ltd.