Identification and Quantification of Bu Shen Yi Sui Capsule by UPLC-LTQ-Orbitrap-MSn and UPLC-QTOF-MS/MS
Identification and Quantification of Bu Shen Yi Sui Capsule by UPLC-LTQ-Orbitrap-MSn and UPLC-QTOF-MS/MS
复制标题
采用 UPLC-LTQ-Orbitrap-MSn 和 UPLC-QTOF-MS/MS 鉴定和定量补肾一髓胶囊。
DOI：
10.1093/chromsci/bmab091
复制
发表时间：
2021-07-21
影响因子：
1.3
通讯作者：
Wang, Lei
中科院分区：
文献类型：
--
作者：
Liu, Haolong;Zhao, Yiyi;Wang, Lei
传统中药因其对特定疾病的显著疗效而被认为是重要的替代疗法。中药配方的特点是分子量大，不同于常规药物。由于中药化学成分的复杂性，适当的质量控制一直是一个巨大的挑战。选择合适的方法对这些化合物进行鉴定和定性是保证其安全性、有效性和质量控制的重要工作。因此，本研究旨在为补肾益髓胶囊（BSYSC）的高分辨LTQ-Orbitrap质谱（UPLC-LTQ-Orbitrap-MSn）鉴定提供新的信息。在此色谱条件下，分离鉴定了80种主要化学成分，主要为蒽醌类、酚酸类和苯乙醇苷类。结合高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF-MS/MS）方法，将其中8个化合物作为BSYSC定量评价的标志化合物。UPLC-LTQ-Orbitrap-MSn和UPLC-QTOF-MS/MS的定性和定量分析为BSYSC的进一步药代动力学研究提供了必要的数据。
Traditional Chinese medicines (TCMs) have been considered as important alternative therapeutics because of their significant medicinal benefits in specific diseases. Chinese herb formula is characterized by a vast molecule that differs in routine medicines. Due to TCMs chemical complexity, proper quality control has been a great challenge. Choosing the appropriate method to identify and qualify these compounds is an important work to ensure its safety, efficacy and quality control. Thus, this study aimed at providing novel information on high-resolution LTQ-Orbitrap mass spectrometer (UPLC-LTQ-Orbitrap-MSn) based identification of Bu Shen Yi Sui capsule (BSYSC), which is used in treating multiple sclerosis as a kind of TCMs. Under the proposed chromatographic conditions, 80 chemical components classified as anthraquinone, phenolic acid and phenylethanoid glycosides were separated and identified from BSYSC. Coupled with the high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) method, eight of them were regarded as marker compounds for the quantitative evaluation of BSYSC. The identification and quantification with precision of UPLC-LTQ-Orbitrap-MSn and UPLC-QTOF-MS/MS could facilitate essential data for further pharmacokinetic studies of BSYSC.