Kif3a interacts with Dynactin subunit p150Glued to organize centriole subdistal appendages
Kif3a interacts with Dynactin subunit p150Glued to organize centriole subdistal appendages
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DOI：
10.1038/emboj.2013.3
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发表时间：
2013-02-20
期刊：
影响因子：
11.4
通讯作者：
Reiter, Jeremy F.
中科院分区：
文献类型：
--
作者：
Kodani, Andrew;Salome Sirerol-Piquer, Maria;Reiter, Jeremy F.
纤毛的形成，微管为基础的结构，在推进和感觉功能，需要Kif 3a，驱动蛋白II的亚基鞭毛内运输（IFT）所必需的。我们已经发现，Kif 3a也需要组织中心粒。在缺乏Kif 3a的情况下，中心粒的近远侧附属物是混乱的，并且缺乏p150（胶合）和Ninein。因此，微管锚定，中心粒凝聚力和基底足的形成被废除的损失Kif 3a。Kif 3a定位于母中心粒并与Dynactin亚基p150（Glued）相互作用。p150（Glued）的耗竭表型模仿了Kif 3a缺失的影响，表明Kif 3a募集p150（Glued）对于近远侧附属物形成至关重要。Kif 3a的转运功能对于近远侧附属器组织是不稳定的，因为缺乏运动活性的Kif 3a的突变形式或运动结构域可以恢复p150（Glued）定位。与缺乏Ift 88的细胞的比较揭示了Kif 3a的中心粒功能独立于IFT。因此，除了它的ciliogenic作用，Kif 3a招募p150（胶合）的近远侧附属物的母亲中心粒，中心体的功能作为微管组织中心的关键。The EMBO Journal（2013）32，597-607. doi：10.1038/daj.2013.3; 2013年2月5日在线发布
Formation of cilia, microtubule-based structures that function in propulsion and sensation, requires Kif3a, a subunit of Kinesin II essential for intraflagellar transport (IFT). We have found that, Kif3a is also required to organize centrioles. In the absence of Kif3a, the subdistal appendages of centrioles are disorganized and lack p150(Glued) and Ninein. Consequently, microtubule anchoring, centriole cohesion and basal foot formation are abrogated by loss of Kif3a. Kif3a localizes to the mother centriole and interacts with the Dynactin subunit p150(Glued) . Depletion of p150(Glued) phenocopies the effects of loss of Kif3a, indicating that Kif3a recruitment of p150(Glued) is critical for subdistal appendage formation. The transport functions of Kif3a are dispensable for subdistal appendage organization as mutant forms of Kif3a lacking motor activity or the motor domain can restore p150(Glued) localization. Comparison to cells lacking Ift88 reveals that the centriolar functions of Kif3a are independent of IFT. Thus, in addition to its ciliogenic roles, Kif3a recruits p150(Glued) to the subdistal appendages of mother centrioles, critical for centrosomes to function as microtubule-organizing centres. The EMBO Journal (2013) 32, 597-607. doi: 10.1038/emboj.2013.3; Published online 5 February 2013