Synthesis of cartilage matrix by mammalian chondrocytes in vitro. I. Isolation, culture characteristics, and morphology.
Synthesis of cartilage matrix by mammalian chondrocytes in vitro. I. Isolation, culture characteristics, and morphology.
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DOI：
10.1083/jcb.93.3.743
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发表时间：
1982-06
期刊：
影响因子：
--
通讯作者：
Schenk RK
中科院分区：
文献类型：
--
作者：
Kuettner KE;Pauli BU;Gall G;Memoli VA;Schenk RK
我们描述了成年牛关节软骨细胞的体外分离和超微结构特征。牛关节软骨切片经过蛋白酶和胶原酶的连续消化以释放细胞。软骨细胞以高密度（1 × 10(5)个细胞/cm2）用Ham's F-12培养基（添加10%胎牛血清）在培养皿或滚轮瓶中镀上。培养前，软骨细胞脱离周围的区域基质。在最初的几天里，他们重新建立了一个领土矩阵。随着时间的推移，软骨细胞可以合成领土和域外基质。基质富含胶原原纤维和钌红阳性蛋白聚糖。这些特征在大量滚轮培养中最为明显，细胞和基质的聚集体表现为长条纹和结节。这种形态揭示了软骨细胞及其基质的组织，与母体关节软骨在体内的组织相似。
We describe the isolation and the ultrastructural characteristics of adult bovine articular chondrocytes in vitro. Slices of bovine articular cartilage undergo sequential digestions with pronase and collagenase in order to release cells. Chondrocytes are plated at high density (1 x 10(5) cells/cm2) in culture dishes or roller bottles with Ham's F-12 medium, supplemented with 10% fetal bovine serum. Before culture, chondrocytes are freed of surrounding territorial matrix. Within the first few days of culture they re-establish a territorial matrix. As time progresses, chondrocytes synthesize both territorial and extraterritorial matrices. The matrices are rich in collagen fibrils and ruthenium red-positive proteoglycans. These features are most apparent in mass roller cultures in which aggregates of cells and matrix appear as long streaks and nodules. This morphology reveals an organization of chondrocytes and their matrices that is similar to that of the parent articular cartilage in vivo.